李桂华

    紫外分光光度法与反相高效液相色谱法测定阿昔洛韦眼用凝胶中阿昔洛韦的含量

    李桂华

    目的 探讨紫外分光光度法及反相高效液相色谱法测定阿昔洛韦眼用凝胶中阿昔洛韦含量的可行性。方法 采用紫外分光光度法、反相高效液相色谱法根据相同方法制备阿昔洛韦眼用凝胶试品后于252 nm波长下测定其阿昔洛韦含量。结果 高效液相法测定阿昔洛韦眼用凝胶中阿昔洛韦含量为(100.49±0.14)%，明显低于紫外分光光度法所测的(101.55±0.47)%，差异有统计学意义(P<0.05)。结论 高效液相色谱方法因可有效分离相关物质，减少杂质干扰，方法简单、结果准确、灵敏度高，相比于紫外分光光度法更利于阿昔洛韦凝胶中阿昔洛韦的含量测定。

    反相高效液相色谱法；阿昔洛韦；眼用凝胶；紫外分光光度法

    【Abstract】Objective To explore the feasibility of the determination of A Silowe in A Silowe ophthalmic gel by UV spectrophotometry and reversed phase high performance liquid chromatography.Methods By UV spectrophotometry，reversed phase high performance liquid chromatography according to the same method of preparing the aciclovir eye gel sample at 252 nm wavelength determination the content of aciclovir.Results Cntent of acyclovir in acyclovir ophthalmic gel(100.49±0.14)% lower than the measured UV spectrophotometry (101.55±0.47)%，the difference was statistically significant(P<0.05).Conclusion HPLC method is effective because of the separation-related substances ......