黄热病病毒检测微阵列的研究制备(英文)
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张海燕 马文丽 郑文岭 广州军区广州总医院医学实验科;南方医科大学分子生物学研究所;
【摘要】目的:制备黄热病病毒寡核苷酸检测微阵列。方法:根据黄热病病毒基因组序列,并应用生物信息学软件设计出寡核苷酸探针用于制备基因芯片,克隆于质粒上的黄热病病毒全长基因DNA经限制性显示技术扩增并标记,完成杂交后对芯片进行扫描和数据分析。结论:微阵列检测技术为检测黄热病病毒提供了一种早期、快速、可靠的方法,具有应用于临床检测的前景。
【关键词】 黄热病病毒 微阵列 寡核苷酸 限制性显示技术
【基金】supported by the National Natural Science Foundation of China(NO.39880032)~~
【分类号】R373
Yellow fever is a viral disease that has caused large epi-demics in tropical regions of sub-Saharan Africa and South Ameri-ca.The disease is caused by the yellow fevervirus(YFV),whichbelongs to the flavivirus group.There are200,000estimated casesof yello
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Yellow fever is a viral disease that has caused large epi-demics in tropical regions of sub-Saharan Africa and South Ameri-ca. The disease is caused by the yellow fever virus (YFV), whichbelongs to the flavivirus group. There are 200,000 estimated casesof yellow fever (with 30,000 deaths) per year. 1-2
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