吴 双 杨红莉 李 浩 孙震晓(北京中医药大学中药学院，北京，100102)

    制何首乌对大鼠肝CYP1A2酶活性及mRNA表达的抑制作用

    吴 双 杨红莉 李 浩 孙震晓

    (北京中医药大学中药学院，北京，100102)

    摘要目的：考察制何首乌水提物对大鼠肝细胞色素P450酶亚型CYP1A2的酶活性及mRNA表达的影响。方法：水回流提取得制何首乌水提物，HPLC确定主要成分并测定其含量。大鼠随机分为空白对照组、制何首乌水提物组(2 g/kg和20 g/kg)，给药组每天按10 mL/kg量灌胃给予制何首乌水提物，连续7 d。取肝分离肝微粒体，HPLC测定肝微粒体对CYP1A2酶的底物非那西丁的代谢消除率，考察肝微粒体CYP1A2酶活性；实时荧光定量PCR技术检测肝组织中CYP1A2基因mRNA的表达。结果：与空白对照组相比，制何首乌水提物20 g/kg组大鼠肝CYP1A2酶活性和mRNA的表达均明显降低(CYP1A2酶活性，P<0. 01；CYP1A2的mRNA表达，P<0. 05)。结论：制何首乌水提物20 g/kg对大鼠肝CYP1A2酶活性和mRNA的表达有明显抑制作用。

    关键词制何首乌水提物；CYP1A2；大鼠肝微粒体；酶活性；mRNA表达

    Prohibitive Effect of Processed Polygonum Multiflorum on CYP1A2 Activities and mRNA Expressions of Rats

    Wu Shuang，Yang Hongli，Li Hao，Sun Zhenxiao

    (College of Chinese Medicine，Beijing University of Chinese Medicine，Beijing 100102，China)

    Abstract Objective：To observe the effects of water extraction of processed Polygonum multiflorum on enzymatic activities and mRNA expressions of CYP1A2 in rats. Methods：The water extraction of processed Polygonum multiflorum was obtained through extraction with water and vacuum drying and the main components were detected by HPLC. Rats were randomly divided into the blank control group and 2 g/kg BW and 20 g/kg BW the water extraction of processed Polygonum multiflorum group. The administration group was continuously given with water extraction of processed Polygonum multiflorum 10 mL/kg a day ......