李秀荣李慧杰许艳艳(山东中医药大学附属医院，济南，5004；山东中医药大学，济南，5004)

    纳米雄黄抑制人乳腺癌MCF-7细胞上皮间质转化的机制探讨

    李秀荣1李慧杰1许艳艳2

    (1山东中医药大学附属医院，济南，250014；2山东中医药大学，济南，250014)

    摘要目的：观察纳米雄黄对乳腺癌MCF-7细胞上皮间质转化的影响，并探讨其相关机制。方法：体外培养乳腺癌MCF-7细胞，不同浓度纳米雄黄溶液干预，并设立对照组，免疫组化实验检测各组细胞E-cad、HIF-1α表达情况，RT-PCR检测SnailmRNA表达情况。结果：各纳米雄黄组E-cad表达阳性率均明显高于对照组(P<0. 01)，而HIF-1α表达阳性率均低于对照组(P<0. 01)；对照组、纳米雄黄低、中、高剂量组、阿霉素组的SnailmRNA相对表达量依次为(52. 16±3. 87)、(30. 10±2. 48)、(47. 08±3. 45)、(41. 42±4. 35)、(36. 07±2. 45)。结论：纳米雄黄可通过上调E-cad表达、下调HIF-1α 及Snail mRNA表达逆转乳腺癌MCF-7细胞上皮间质转化发挥抗肿瘤转移作用。

    关键词纳米雄黄；乳腺癌；上皮间质转化；肿瘤转移

    The Mechanism of Nanometer Realgar to Inhibit the Epithelial Mesenchymal Transition of Breast Cancer MCF-7 cells

    Li Xiurong1，Li Huijie1，Xu Yanyan2

    (1 The Affiliated Hospital of Shandong University of Traditional Chinese Medicine，Ji'nan 250014，China；2 Shandong University of Traditional Chinese Medicine，Ji'nan 250014，China)

    Abstract Objective：To explore the effect of nanometer realgar affecting epithelial mesenchymal transition of the breast cancer MCF-7 cells and its possible mechanism. Methods：Breast cancer MCF-7 cells were cultured in vitroand and use different doses of nanometer realgar to intervene. A control group was made. To test the expression of E-cad and HIF-1α ......