刺络泻血对高尿酸血症大鼠血尿酸及相关酶活性的影响(1)
摘要 目的：观察刺络泻血疗法对高尿酸血症模型大鼠血尿酸及相关酶活性的影响并探讨其机制。方法：将40只清洁级SD大鼠分为正常组、模型组、别嘌呤醇组及刺络泻血组，每组10只，以腺嘌呤、盐酸乙胺丁醇混合液灌胃造模。第4周造模成功后，别嘌呤醇组按照0.03 g/(kg·d)的剂量给大鼠灌胃别嘌呤醇，2次/周；刺络泻血组选取大鼠委中穴及足三里穴穴位附近的血络，出血0.3～0.5 mL，至第8周。第9周后腹主动脉取血，采用磷钨酸法测定血尿酸，采用酶比色法测定黄嘌呤氧化酶(XOD)和腺苷脱氨酶(ADA)的含量。结果：实验结束后，与正常组相比，模型组SUA、ADA、XOD含量均显著增高(P<0.05)，差异有统计学意义；与模型组相比，别嘌呤醇组SUA、XOD含量显著降低(P<0.05)，刺络泻血组SUA、ADA、XOD含量差异均有统计学意义(P<0.05)。结论：刺络泻血疗法可以有效抑制XOD、ADA活性，降低高尿酸血症大鼠SUA水平。
关键词 刺络泻血；高尿酸血症；血尿酸；黄嘌呤氧化酶；腺苷脱氨酶
Effects of Blood Pricking Therapy on SUA and the Activity of Related Enzyme in Hyperuricemic Rats
Tan Li， Wang Ning， Zhao Huiling， Zhan Huimin， Chen Zichen， Wei Danlei， Zeng Rui， Zhang Bo
(Beijing University of Chinese Medicine， Beijing 100029， China)
Abstract Objective：To observe the effects of blood pricking therapy on serum uric acid (SUA) and the activity of related enzyme in hyperuricemic rats， and explore its mechanism. Methods：A total of 40 male SD rats were randomly divided into four groups：normal group (10 rats)， model group (10 rats)， allopurinol group (10 rats)， and blood pricking group (10 rats). Rats with hyperuricemic were modeling by adenine and ethambutol hydrochloride intragastrically. Four weeks after successful modeling， rats in allopurinol group were lavaged allopurinol according to the body weight of rats 0.03 g/L (kg·d)， 2 times/week； rats in blood pricking group were pricked for bloodletting 0.3～0.5 mL on the rats leg collaterals of the Bladder Meridian of the FootTaiyang and Kidney Meridian of FootShaoyin until the eighth week. At the end of eighth week， the rats were made anesthesia with 10% of chloral hydrate， then 5 mL blood was draw from the abdominal aorta. By phosphoric acid method， the SUA were tested， and with enzymecolorimetric method， the activity of the xanthine oxidase (XOD) and adenosine deaminase(ADA)were tested. Results：Compared with the normal group， the levels of SUA， ADA and XOD in the model group were significantly increased (P<0.05)； compared with the model group， the levels of SUA and XOD in the allopurinol group were significantly reduced (P<0.05)； the changes of SUA， ADA and XOD in the blood pricking group were significant (P<0.05). Conclusion：The blood pricking therapy can significantly inhibit the activity of XOD and ADA， reducing the level of SUA in rats with hyperuricemia.
Key Words Blood pricking therapy； Hyperuricemic； SUA； XOD； ADA
中圖分类号：R245文献标识码：Adoi：10.3969/j.issn.1673-7202.2017.03.039
高尿酸血症(Hyperuricemia，HUA)是嘌呤代谢障碍引起的代谢性疾病，国际上将HUA的诊断标准定义为在正常嘌呤饮食状态下，非同日两次空腹血尿酸水平男>420 μmol/L(7 mg/dL)，女>360 μmol/L(6 mg/dL)，未发作为痛风的HUA称为无症状HUA[1]。多项流行病学和临床研究结果证实，HUA与痛风、心血管疾病、肾脏疾病、代谢综合征等疾病的发生、发展密切相关[24]。HUA已经成为严重威胁人类健康的代谢性疾病，受到了医学界的广泛重视。目前临床上很多患者除了表现为尿酸水平升高外，并未表现出其他明显临床症状，少数患者可以发展为痛风，出现急性关节炎、痛风肾和痛风石等临床症状和阳性体征，在正常生理情况下，血尿酸浓度﹥408 μmol/L(6.8 mg/dL)时会引起尿酸盐结晶，约10%的高尿酸血症患者最终会出现痛风症状[56]。有研究表明，高尿酸血症患者的血尿酸水平应至少控制在6 mg/dL以下，低于5 mg/dL更理想[78]。刺络泻血疗法是一种操作简单、安全有效、不良反应小的中医传统疗法，研究表明，刺络泻血疗法可以有效降低高尿酸患者血清尿酸水平[9]。我们在本实验采用腺嘌呤和盐酸乙胺丁醇灌胃以建立大鼠高尿酸模型，观察刺络泻血对高尿酸模型大鼠血清尿酸(Serum Uric Acid，SUA)及黄嘌呤氧化酶(Xanthine Oxidase，XOD)、腺苷脱氨酶(Adenosine Deaminase，ADA)活性的影响，以了解刺络泻血疗法在治疗高尿酸血症的效应并探讨其可能作用机制，为临床上刺络泻血疗法治疗高尿酸血症提供理论依据。, http://www.100md.com(谭丽 王宁 赵慧玲 战慧敏 陈子晨 魏丹蕾 曾蕊 张博)
关键词 刺络泻血；高尿酸血症；血尿酸；黄嘌呤氧化酶；腺苷脱氨酶
Effects of Blood Pricking Therapy on SUA and the Activity of Related Enzyme in Hyperuricemic Rats
Tan Li， Wang Ning， Zhao Huiling， Zhan Huimin， Chen Zichen， Wei Danlei， Zeng Rui， Zhang Bo
(Beijing University of Chinese Medicine， Beijing 100029， China)
Abstract Objective：To observe the effects of blood pricking therapy on serum uric acid (SUA) and the activity of related enzyme in hyperuricemic rats， and explore its mechanism. Methods：A total of 40 male SD rats were randomly divided into four groups：normal group (10 rats)， model group (10 rats)， allopurinol group (10 rats)， and blood pricking group (10 rats). Rats with hyperuricemic were modeling by adenine and ethambutol hydrochloride intragastrically. Four weeks after successful modeling， rats in allopurinol group were lavaged allopurinol according to the body weight of rats 0.03 g/L (kg·d)， 2 times/week； rats in blood pricking group were pricked for bloodletting 0.3～0.5 mL on the rats leg collaterals of the Bladder Meridian of the FootTaiyang and Kidney Meridian of FootShaoyin until the eighth week. At the end of eighth week， the rats were made anesthesia with 10% of chloral hydrate， then 5 mL blood was draw from the abdominal aorta. By phosphoric acid method， the SUA were tested， and with enzymecolorimetric method， the activity of the xanthine oxidase (XOD) and adenosine deaminase(ADA)were tested. Results：Compared with the normal group， the levels of SUA， ADA and XOD in the model group were significantly increased (P<0.05)； compared with the model group， the levels of SUA and XOD in the allopurinol group were significantly reduced (P<0.05)； the changes of SUA， ADA and XOD in the blood pricking group were significant (P<0.05). Conclusion：The blood pricking therapy can significantly inhibit the activity of XOD and ADA， reducing the level of SUA in rats with hyperuricemia.
Key Words Blood pricking therapy； Hyperuricemic； SUA； XOD； ADA
中圖分类号：R245文献标识码：Adoi：10.3969/j.issn.1673-7202.2017.03.039
高尿酸血症(Hyperuricemia，HUA)是嘌呤代谢障碍引起的代谢性疾病，国际上将HUA的诊断标准定义为在正常嘌呤饮食状态下，非同日两次空腹血尿酸水平男>420 μmol/L(7 mg/dL)，女>360 μmol/L(6 mg/dL)，未发作为痛风的HUA称为无症状HUA[1]。多项流行病学和临床研究结果证实，HUA与痛风、心血管疾病、肾脏疾病、代谢综合征等疾病的发生、发展密切相关[24]。HUA已经成为严重威胁人类健康的代谢性疾病，受到了医学界的广泛重视。目前临床上很多患者除了表现为尿酸水平升高外，并未表现出其他明显临床症状，少数患者可以发展为痛风，出现急性关节炎、痛风肾和痛风石等临床症状和阳性体征，在正常生理情况下，血尿酸浓度﹥408 μmol/L(6.8 mg/dL)时会引起尿酸盐结晶，约10%的高尿酸血症患者最终会出现痛风症状[56]。有研究表明，高尿酸血症患者的血尿酸水平应至少控制在6 mg/dL以下，低于5 mg/dL更理想[78]。刺络泻血疗法是一种操作简单、安全有效、不良反应小的中医传统疗法，研究表明，刺络泻血疗法可以有效降低高尿酸患者血清尿酸水平[9]。我们在本实验采用腺嘌呤和盐酸乙胺丁醇灌胃以建立大鼠高尿酸模型，观察刺络泻血对高尿酸模型大鼠血清尿酸(Serum Uric Acid，SUA)及黄嘌呤氧化酶(Xanthine Oxidase，XOD)、腺苷脱氨酶(Adenosine Deaminase，ADA)活性的影响，以了解刺络泻血疗法在治疗高尿酸血症的效应并探讨其可能作用机制，为临床上刺络泻血疗法治疗高尿酸血症提供理论依据。, http://www.100md.com(谭丽 王宁 赵慧玲 战慧敏 陈子晨 魏丹蕾 曾蕊 张博)
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