和解泄热安神法对乙醇依赖症大鼠戒断行为的干预研究(1)
摘要 目的：研究和解泄熱安神法方药对乙醇依赖症大鼠戒断行为的干预作用。方法：21只雄性SD大鼠用随机数字表随机分为正常组、观察组和对照组，每组7只，除正常组大鼠外，其余均采用饮用含乙醇饮水(6%，v/v)21 d复制乙醇依赖症模型。之后采用和解泄热安神方药灌胃观察组、纯净水灌胃对照组大鼠共7天，灌胃期间仍饮用含乙醇饮水。第29天起撤除含乙醇饮水，所有大鼠均饮用纯净水，观察记录大鼠乙醇戒断行为。整个实验期间观察大鼠精神状态、活动、记录周体重变化及日饮水量。结果：随着乙醇依赖大鼠的戒断时间延长，戒断行为评分逐渐升高，与正常组比较，对照组的戒断行为评分明显升高(P<0.05)，于戒断后6 h行为表现最明显并持续至24 h。观察组行为评分介于二者之间，并明显低于对照组(P<0.05)。结论：和解泄热安神法方药可以减轻乙醇依赖症大鼠的戒断行为评分，缓解乙醇戒断症状。
关键词 中医药；和解泄热安神法；乙醇依赖症；乙醇戒断；大鼠
Interventional Study on Harmonizing， Heat-relieving and Mind-tranquilizing Therapy in Treating Alcohol Withdrawal Behaviors in Alcohol Dependence Model Rats
, http://www.100md.com
Zhang Xiaoxia1， Yang Ling2， Xue Xiaoxuan2， Yu Yiqun1， Wang Zhibin1
(1 Gastroenterology Department， Dongfang Hospital Affiliated to Beijing University of Chinese Medicine， Beijing 100078， China； 2 Functional Inspection Department， Dongfang Hospital Affiliated to Beijing University of Chinese Medicine， Beijing 100078， China)
Abstract Objective：To observe the interventional effects of harmonizing， heat-relieving and mind-tranquilizing therapy in treating alcohol withdrawal behaviors in alcohol dependence model rats.Methods：Twenty-one male SD rats were randomly divided into normal， treatment and control group by random number table with 7 rats in each group. All rats were fed water with alcohol (6%， v/v) for 21 days to make alcohol dependence models except normal ones. Rats in the treatment group were administered by medicinal granules with the functions of harmonizing， heat-relieving and mind-tranquilizing while rats in the control group were fed with pure water for constantly 7 days， and rats drank water with alcohol during the experiment. From the 29th day， all rats started to drink pure water without alcohol， and their behaviors， mental status， life activity， weekly body weight and daily drinking were observed and evaluated.Results：With the extension of alcohol withdrawal time， sores of withdrawal behaviors raised gradually. The scores of control group was higher when compared with normal group (P<0.05). It had a peak score at 6 h and lasted until 24 h after withdrawal. The sore of the treatment group was between that of the normal and control group， which was lower than that of the control group (P<0.05).Conclusion：Harmonizing， heat-relieving and mind-tranquilizing therapy may decrease scores of alcohol withdrawal in alcohol dependence rats and relieve alcohol withdrawal symptoms.
Key Words Traditional Chinese Medicine； Harmonizing， heat-relieving and mind-tranquilizing therapy； Alcohol dependence； Alcohol withdrawal； Rats, 百拇医药(张晓霞 杨玲 薛晓轩 余轶群 王志斌)
关键词 中医药；和解泄热安神法；乙醇依赖症；乙醇戒断；大鼠
Interventional Study on Harmonizing， Heat-relieving and Mind-tranquilizing Therapy in Treating Alcohol Withdrawal Behaviors in Alcohol Dependence Model Rats
, http://www.100md.com
Zhang Xiaoxia1， Yang Ling2， Xue Xiaoxuan2， Yu Yiqun1， Wang Zhibin1
(1 Gastroenterology Department， Dongfang Hospital Affiliated to Beijing University of Chinese Medicine， Beijing 100078， China； 2 Functional Inspection Department， Dongfang Hospital Affiliated to Beijing University of Chinese Medicine， Beijing 100078， China)
Abstract Objective：To observe the interventional effects of harmonizing， heat-relieving and mind-tranquilizing therapy in treating alcohol withdrawal behaviors in alcohol dependence model rats.Methods：Twenty-one male SD rats were randomly divided into normal， treatment and control group by random number table with 7 rats in each group. All rats were fed water with alcohol (6%， v/v) for 21 days to make alcohol dependence models except normal ones. Rats in the treatment group were administered by medicinal granules with the functions of harmonizing， heat-relieving and mind-tranquilizing while rats in the control group were fed with pure water for constantly 7 days， and rats drank water with alcohol during the experiment. From the 29th day， all rats started to drink pure water without alcohol， and their behaviors， mental status， life activity， weekly body weight and daily drinking were observed and evaluated.Results：With the extension of alcohol withdrawal time， sores of withdrawal behaviors raised gradually. The scores of control group was higher when compared with normal group (P<0.05). It had a peak score at 6 h and lasted until 24 h after withdrawal. The sore of the treatment group was between that of the normal and control group， which was lower than that of the control group (P<0.05).Conclusion：Harmonizing， heat-relieving and mind-tranquilizing therapy may decrease scores of alcohol withdrawal in alcohol dependence rats and relieve alcohol withdrawal symptoms.
Key Words Traditional Chinese Medicine； Harmonizing， heat-relieving and mind-tranquilizing therapy； Alcohol dependence； Alcohol withdrawal； Rats, 百拇医药(张晓霞 杨玲 薛晓轩 余轶群 王志斌)