黄芩苷介导光动力疗法治疗宫颈癌的疗效及其机制研究(1)
摘要 目的：觀察黄芩苷介导光动力疗法(PDT)治疗宫颈癌的疗效，探讨其可能机制。方法：将宫颈癌Hela细胞悬液注入裸鼠皮下，构建移植瘤模型，成模后随机均匀分为模型组、黄芩苷-PDT观察组、PDT观察组、姜黄素-PDT观察组4组，每组18只。PDT观察组：肿瘤周围注射100 μL生理盐水，6 h后瘤周注射硼砂缓冲液100 μL后行PDT治疗，照射剂量为80 J/cm2(435 nm波长，0.60 A，0.54 W，距离肿瘤表面3～5 cm，照射2 min 28 s);黄芩苷-PDT观察组：以80 μg/mL的黄芩苷溶液100 μL代替生理盐水，其他同PDT观察组;姜黄素-PDT观察组：以50 μmol/L的姜黄素溶液100 μL代替生理盐水，其他同PDT观察组;模型组：以自然光照代替PDT治疗，其他同PDT观察组。治疗1 d后，各组处死6只取材肿瘤组织制备成病理切片，HE染色观察组织病理学改变;另外处死6只，利用蛋白质印迹法检测肿瘤组织中Bcl-2和Bax蛋白的表达情况;剩余6只分别于治疗第1、3、7、14 d测量肿瘤体积，计算肿瘤体积抑瘤率。结果：模型组肿瘤体积持续增大，光动力治疗1 d后肿瘤组织出现坏死，肿瘤生长受到抑制，黄芩苷-PDT观察组和姜黄素-PDT观察组肿瘤体积在3 d内均无增大趋势，随后逐渐增大。黄芩苷-PDT观察组Bcl-2和Bax蛋白表达与模型组比较，差异有统计学意义(P<0.05)。结论：黄芩苷介导的PDT能够有效抑制宫颈癌体外移植瘤的增长，其机制可能通过促进癌细胞凋亡发挥作用。
关键词 黄芩苷;姜黄素;光动力疗法;肿瘤抑制;凋亡机制
Abstract Objective：To observe the therapeutic effects of baicalin-mediated photodynamic therapy (PDT) on cervical cancer and explore its possible mechanism. Methods：A cervical cancer Hela cell suspension was injected subcutaneously into nude mice to construct a transplanted tumor model. After the model was formed， it was randomly and evenly divided into 4 groups： a model group， a baicalin-PDT treatment group， a PDT treatment group， and a curcumin-PDT treatment group ， with 18 cases in each group. PDT treatment group： 100μL of saline was injected around the tumor， and 100 μL of borax buffer was injected around the tumor 6 hours later， followed by PDT treatment. The irradiation dose was 80J/cm2 (435nm wavelength， 0.60A， 0.54W， 3-5cm from the tumor surface， irradiation 2 min28s); Baicalin-PDT treatment group： 100 μL of baicalin solution at 80 μg/mL instead of normal saline， others were the same as PDT treatment group; curcumin-PDT treatment group： 50 μmol/L curcumin solution instead of 100 ml normal saline， others were the same as PDT treatment group; model group： natural light instead of PDT treatment， others were the same as PDT treatment group. After 1 day of treatment， 6 rats were sacrificed in each group and their tumor tissues were prepared for pathological sections， and the histopathological changes were observed by HE staining; another 6 were sacrificed， and the expression of Bcl-2 and Bax proteins in tumor tissues were detected by Western blot technology; their tumor volume of 6 remaining rats， was measured only on the 1st， 3rd， 7th， and 14th days of treatment， and the tumor volume inhibition rate was also calculated. Results：The tumor volume in the model group continued to increase. Tumor tissue appeared necrosis and tumor growth was inhibited after 1 day of photodynamic therapy. The tumor volume of baicalin-PDT treatment group and curcumin-PDT treatment group did not increase within 3 days， and then gradually increased. The expression of Bcl-2 and Bax proteins in the baicalin-PDT treatment group was significantly different from that in the model group (P<0.01). Conclusion：Baicalin-mediated PDT can effectively inhibit the growth of cervical cancer xenografts in vitro， and its mechanism may play a role in promoting cancer cell apoptosis., 百拇医药(郭婕 潘彦舒 庞大承 牟天龙)
关键词 黄芩苷;姜黄素;光动力疗法;肿瘤抑制;凋亡机制
Abstract Objective：To observe the therapeutic effects of baicalin-mediated photodynamic therapy (PDT) on cervical cancer and explore its possible mechanism. Methods：A cervical cancer Hela cell suspension was injected subcutaneously into nude mice to construct a transplanted tumor model. After the model was formed， it was randomly and evenly divided into 4 groups： a model group， a baicalin-PDT treatment group， a PDT treatment group， and a curcumin-PDT treatment group ， with 18 cases in each group. PDT treatment group： 100μL of saline was injected around the tumor， and 100 μL of borax buffer was injected around the tumor 6 hours later， followed by PDT treatment. The irradiation dose was 80J/cm2 (435nm wavelength， 0.60A， 0.54W， 3-5cm from the tumor surface， irradiation 2 min28s); Baicalin-PDT treatment group： 100 μL of baicalin solution at 80 μg/mL instead of normal saline， others were the same as PDT treatment group; curcumin-PDT treatment group： 50 μmol/L curcumin solution instead of 100 ml normal saline， others were the same as PDT treatment group; model group： natural light instead of PDT treatment， others were the same as PDT treatment group. After 1 day of treatment， 6 rats were sacrificed in each group and their tumor tissues were prepared for pathological sections， and the histopathological changes were observed by HE staining; another 6 were sacrificed， and the expression of Bcl-2 and Bax proteins in tumor tissues were detected by Western blot technology; their tumor volume of 6 remaining rats， was measured only on the 1st， 3rd， 7th， and 14th days of treatment， and the tumor volume inhibition rate was also calculated. Results：The tumor volume in the model group continued to increase. Tumor tissue appeared necrosis and tumor growth was inhibited after 1 day of photodynamic therapy. The tumor volume of baicalin-PDT treatment group and curcumin-PDT treatment group did not increase within 3 days， and then gradually increased. The expression of Bcl-2 and Bax proteins in the baicalin-PDT treatment group was significantly different from that in the model group (P<0.01). Conclusion：Baicalin-mediated PDT can effectively inhibit the growth of cervical cancer xenografts in vitro， and its mechanism may play a role in promoting cancer cell apoptosis., 百拇医药(郭婕 潘彦舒 庞大承 牟天龙)
参见：首页 > 医疗版 > 疾病专题 > 妇产科 > 女性生殖器官肿瘤及肿瘤样病变 > 子宫颈癌