薛刚 刘杰 许利军 石建 凌博凡

    摘要 目的：研究扶正消积胶囊在体外对人胃癌细胞BGC-823增殖抑制及凋亡的影响。方法：MTT实验检测扶正消积胶囊对BGC-823细胞增殖的影响;流式细胞仪检测其对BGC-823细胞凋亡的影响;Real Time-PCR、Western blotting实验检测其凋亡相关基因表达。结果：与空白组比较，BGC-823细胞的增殖能被扶正消积胶囊显著抑制(给药24 h)，此抑制率随给药剂量的增加而上升，在剂量为5 mg/ mL时，其抑制率达86.71%，且这种抑制呈剂量依赖关系。Annexin V/PI双染法联合流式细胞仪结果显示扶正消积胶囊能诱导BGC-823细胞凋亡，BGC-823细胞早期凋亡率随给药剂量的增加，在给药剂量分别为2.5 mg/mL、5 mg/mL时，BGC-823细胞早期凋亡率分别达到15.76%、38.25%;Western blotting、Real Time-PCR结果表明，Procaspase-3、9蛋白酶表达随剂量的增加而明显减小，Bcl-2、Bcl-xl、Survivin 3种基因表达随给药剂量的增加而下降，Bax基因表达则相反。结论：扶正消积胶囊能通过激活caspase级联反应抑制胃癌细胞增殖并诱导其凋亡。

    关键词 扶正消积胶囊;BGC-823胃癌细胞;肿瘤;增殖;凋亡

    Abstract Objective：To investigate the influence of Fuzheng Xiaoji Capsule on the proliferation inhibition of human gastric cancer cell BGC-823 and its apoptosis.Methods：Effects of Fuzheng Xiaoji Capsule on proliferation of BGC-823 cells were investigated with MTT assay.Flow cytometry was used to detect the cell apoptosis while the effect of apoptosis related gene of Fuzheng Xiaoji capsule on BGC-823 cell was measured by real time-PCR and Western blotting.Results：MTT assay showed that the BGC-823 cells were inhibited by Fuzheng Xiaoji Capsule compared with control group ......