陆茂等

    [摘要] 目的 观察PI-103在体外对人黑色素瘤A375细胞迁移和侵袭能力的影响，并探讨可能的作用机制。 方法 应用MTT法检测不同浓度PI-103对A375细胞增殖的影响，Transwell小室实验检测0.1、0.2 μmol/L PI-103对A375细胞迁移能力和侵袭能力的影响，Western Blot法测定细胞基质金属蛋白酶(MMP)-2、MMP-9的表达。 结果 0.1、0.2 μmol/L PI-103对A375细胞增殖的抑制作用不明显(P > 0.05)；在浓度高于0.4 μmol/L时，PI-103能够明显抑制A375细胞的增殖(P < 0.05)。0.1 μmol/L PI-103作用后，迁移实验和运动实验穿膜细胞数分别为(91.73±13.80)、(63.67±8.54)，与对照组比较明显减少，差异有统计学意义(P < 0.05)；0.2 μmol/L PI-103作用后，迁移实验和运动实验穿膜细胞数分别为(64.07±9.22)、(34.80±7.89)，与对照组比较明显减少，差异有统计学意义(P < 0.05)。0.1、0.2 μmol/L PI-103作用后，A375细胞MMP-2、MMP-9蛋白表达明显低于对照组，差异有统计学意义(P < 0.05)。 结论 PI-103可通过下调MMP-2、MMP-9的表达抑制A375细胞迁移和侵袭，为其应用于抗恶性黑素瘤转移治疗提供了实验依据。

    [关键词] PI-103；黑色素瘤；迁移；侵袭

    [中图分类号] R739.5 [文献标识码] A [文章编号] 1673-7210(2014)09(a)-0014-04

    [Abstract] Objective To investigate the effects of PI-103 on the migratory and invasive abilities of human melanoma A375 cells in vitro and its mechanism. Methods The effects of PI-103 with different concentration on the proliferation of A375 cells were detected by MTT assay. The effects of PI-103 at 0.1， 0.2 μmol/L concentration on the migratory and invasive abilities of A375 cells were detected by transwell chamber assay. The expressions of MMP-2 and MMP-9 in A375 cells were determined by Western Blot. Results The inhibition effects on the proliferation of A375 cells treated with PI-103 at 0.1 ......