温昱 李彬

    [摘要] 目的 探讨体外诱导小鼠骨髓CD45-细胞向窦房结起搏样细胞定向分化的方法。 方法 免疫磁珠分选小鼠骨髓CD45-细胞，体外培养扩增后，采用添加了5-氮胞苷和小鼠窦房结组织培养上清液的诱导培养基培养，经免疫荧光染色检测起搏样细胞标志物神经丝蛋白-160(NF-160)和超级化激活环核苷酸门控阳离子通道(HCN)4表达，并采用qRT-PCR定量检测起搏基因NF-160、HCN4和HCN2表达。 结果 小鼠骨髓CD45-细胞经体外诱导培养后得到的分化细胞表达NF-160和HCN4；qRT-PCR分析显示，与体外培养的小鼠窦房结细胞比较，分化起搏样细胞NF-160和HCN4的表达升高，HCN2的表达降低。 结论 体外培养能够将小鼠骨髓CD45-细胞诱导分化为窦房结起搏样细胞，为构建生物起搏器种子细胞提供选择。

    [关键词] 起搏样细胞；CD45；HCN4；NF-160；窦房结

    [中图分类号] R329.2 [文献标识码] A [文章编号] 1673-7210(2015)06(b)-0009-04

    Inducing directional differentiation of mouse bone marrow CD45-cells into sinoatrial node pacemaker-like cells in vitro

    WEN Yu1 LI Bin2

    1.Department of Histology & Embryology， Basic Medical College of China Medical University， Liaoning Province， Shenyang 110122， China； 2.Department of Sports Medicine， Affiliated Shengjing Hospital of China Medical University， Liaoning Province， Shenyang 110022， China

    [Abstract] Objective To study method on inducing directional differentiation of mouse bone marrow CD45-cells into sinoatrial node pacemaker-like cells in vitro. Methods Mouse bone marrow CD45-cells were derived by using immunomagnetic beads system. After amplification in vitro ......