韩晓珂 梁朝锋 祁 俊

    安徽省芜湖市中医医院制剂室，安徽芜湖 241000

    十七味大活血胶囊的质量标准研究

    韩晓珂梁朝锋祁俊

    安徽省芜湖市中医医院制剂室，安徽芜湖241000

    目的建立十七味大活血胶囊的质量标准。方法显微法对延胡索进行鉴别；薄层色谱法对三七、大黄进行鉴别；HPLC法测定丹酚酸B、苦杏仁苷的含量。丹酚酸B色谱条件为：以C18为色谱柱，流动相为乙腈-0.1%甲酸溶液(22∶78)；检测波长为286 nm；苦杏仁苷色谱条件为：以C18为色谱柱，流动相为甲醇-水(20∶80)；检测波长为210 nm。结果 显微鉴别粉末特征明显；薄层鉴别斑点清晰，阴性无干扰；丹酚酸B在17.23～172.3 μg/mL范围内线性关系良好(r=1)，平均回收率为97.9%，RSD为1.1%；苦杏仁苷在11.04～110.38 μg/mL范围内线性关系良好(r=0.9998)，平均回收率为100.1%，RSD为1.5%。三批样品中两种成分的平均含量分别为1.57、0.41 mg/粒。结论该法准确、简单，适用于十七味大活血胶囊的质量控制。

    十七味大活血胶囊；显微鉴别；薄层色谱；丹酚酸B；苦杏仁苷

    [Abstract]Objective To estab1ish the qua1ity contro1 of Shiqiweidahuoxue Capsu1es.Methods The microscopica1 identification was adopted to ana1yze rizoma coryda1is.TLC method was used to identity radix notoginseng and rhubarb.The contents of s1viano1ic acid B and amygda1in were determined by HPLC method.Sviano1ic acid B was separated on C18co1umn with acetonitri1e-0.1%formic acid so1ution(22∶78)as mobi1e phase，detection wave1ength was 286 nm.Amygda1in was separated on C18co1umn with methano1-water(20∶80)as mobi1e phase，detection wave1ength was 210 nm. Results Microscopic identification had powder characteristics of Rhizoma Coryda1is.The spots were c1ear without interference in the b1ank reference.The 1inear ranges of sa1viano1ic acid B and amygda1in were 17.23-172.3 μg/mL(r=1) ......