肖喜刚谢德轩孙 博刘宗泓

    1.哈尔滨医科大学附属第一医院CT室，黑龙江哈尔滨150001；2.哈尔滨医科大学附属第一医院心血管外科，黑龙江哈尔滨150001

    外科手术建立犬Stanford B型急性主动脉夹层动物模型

    肖喜刚1谢德轩1孙 博2刘宗泓2

    1.哈尔滨医科大学附属第一医院CT室，黑龙江哈尔滨150001；2.哈尔滨医科大学附属第一医院心血管外科，黑龙江哈尔滨150001

    目的探讨通过外科开胸手术制备犬Stanford B型急性主动脉夹层模型的方法。方法选取健康2～3年的比格犬5只，术前进行高脂饲料喂养8周。对实验犬进行静脉麻醉后，切开肋间肌、胸膜，逐步暴露主动脉，在距左锁骨下动脉远端2～3 cm处用0T针斜刺入降主动脉中膜，用冠脉刀迅速切开主动脉管壁全层后，用球囊导管分离主动脉的中膜和内膜，之后用5-0 prolene线缝合动脉外膜和中膜，静脉注射肾上腺素升高血压，术后24 h内对实验犬进行多次CT血管成像，动态观察采集夹层的影像学信息。结果5只实验犬中，共有3只动物术后形成主动脉夹层，CTA图像显示均显示了真假双腔，根据主动脉夹层的Stanford分型标准，确定形成了Stanford B型主动脉夹层。结论外科手术是建立犬Stanford B型主动脉夹层模型的可靠方法。

    主动脉夹层；外科手术；Stanford B型；动物模型

    [Abstract]Objective To investigate the surgical method for producing Stanford B acute aortic dissection model in canine.Methods Five healthy dogs of 2 to 3 years with preoperative high-fat diet for 8 weeks were selected，after intravenous anesthesia，the intercostal muscle and pleura were cut，and the aorta was exposed gradually，0T needle was punctured obliquely into the membrane of descending aorta at the position 2-3 cm away from the left subclavian.Intima and media were seperated by the mechanical force using the balloon catheter ......