付文娟 董桂兰 孙芳初 王连立 郭静

    [摘要] 目的 探讨伴有kazal域的富含半胱氨酸的逆转诱导蛋白(RECK)与基质金属蛋白酶9(MMP-9)在人骨肉瘤组织中的表达及相关性。 方法 选取唐山市人民医院2001年1月～2010年12月临床及病理资料完整存档的人骨肉瘤组织标本34例为观察组，另取临床及病理资料完整存档的骨软骨瘤组织标本34例为对照组，采用免疫组织化学法检测RECK和MMP-9，采用定量PCR法检测RECK和MMP-9的mRNA，并分析指标相关性。 结果 观察组RECK阳性率(58.8%)低于对照组(91.2%)，而MMP-9阳性率(64.7%)高于对照组(11.8%)，差异有统计学意义(P 0.05)。RECK阳性率、RECK的mRNA表达、MMP-9阳性率、MMP-9的mRNA表达在转移、复发以及生存时间方面比较，差异有统计学意义(P < 0.05)。RECK与人骨肉瘤组织呈明显负相关(r=-0.561，P=0.000)。MMP-9与人骨肉瘤组织呈明显正相关(r=0.613，P=0.000)。 结论 RECK可抑制MMP-9表达，发挥抑癌作用，有望成为未来诊断以及治疗人骨肉瘤的潜在靶標。

    [关键词] 伴有kazal域的富含半胱氨酸的逆转诱导蛋白；基质金属蛋白酶9；人骨肉瘤；相关性

    [中图分类号] R73-3 [文献标识码] A [文章编号] 1673-7210(2016)11(c)-0016-05

    Expression and correlation analysis of RECK and MMP-9 in human osteosarcoma tissues

    FU Wenjuan1 DONG Guilan1 SUN Fangchu1 WANG Lianli1 GUO Jing2

    1.The Fifth Department of Radiotherapy and Chemotherapy， People's Hospital of Tangshan City， Hebei Province， Tangshan 063000， China；2.Medical Experimental Research Center， North China University of Science and Technology， Hebei Province， Tangshan 063000，China

    [Abstract] Objective To explore the expression and correlation analysis of RECK and MMP-9 in human osteosarcoma tissues. Methods 34 cases of human osteosarcoma tissues which had complete clinical and pathological data were selected in Tangshan People's Hospital from January 2001 to December 2010 as observation group. 34 cases of osteochondroma tissues which had complete clinical and pathological data were selected as control group. Immunohistochemical method was used to detect RECK and MMP-9. Quantitative PCR method was used to detect mRNA of RECK and MMP-9. Correlation of indexes were analyzed. Results Positive rate of RECK in observation group (58.8%) was lower than that of control group (91.2%) ......