赵祥升 杨美华 弓宝

    [摘要] 目的 建立同时测定酸枣仁中6种成分含量的高效液相色谱-蒸发光检测器(HPLC-ELSD)法。 方法 经70%乙醇超声提取80 min，采用Kromasil C18(250 mm×4.6 mm，5.0 μm)色谱柱进行分离，以乙腈-0.1%乙酸为流动相，梯度洗脱，流速为1.0 mL/min，柱温35℃；ELSD检测，载气流速为2.9 mL/min，漂移管温度为100℃，测定酸枣仁中斯皮诺素、酸枣仁皂苷A、酸枣仁皂苷D、酸枣仁皂苷B、酸枣仁皂苷B1和白桦脂酸6种成分的含量。回收率计算采用外标法，按峰面积计算。 结果 斯皮诺素、酸枣仁皂苷A、酸枣仁皂苷D、酸枣仁皂苷B、酸枣仁皂苷B1和白桦脂酸在各自质量浓度范围内与峰面积呈良好的线性关系(R2>0.9991)；平均加样回收率为94.91%～99.35%，RSD为0.78%～1.23%。不同产地酸枣仁中6种成分的含量差异较大，斯皮诺素和白桦脂酸的含量最高，3批样品中检测到酸枣仁皂苷B1。 结论 该方法简便、准确、重现性好，适用于酸枣仁中多成分的同时快速检测。

    [关键词] 酸枣仁；多成分测定；高效液相色谱-蒸发光检测器

    [中图分类号] R917 [文献标识码] A [文章编号] 1673-7210(2016)12(c)-0149-05

    [Abstract] Objective To establish a HPLC-ELSD method for simultaneous determination of six compounds in ZiziphiSpinosae Semen. Methods The sample was extracted by ultrasonication for 80 min using 70% ethanol and the target compounds were separated on a Kromasil C18 chromatographic column (250 mm×4.6 mm， 5.0 μm). A mixture of acetonitrile-water containing 0.1% acetic acid was utilized as the mobile phase at the flow rate of 1.0 mL/min， and the column temperature was set 35℃. The drift tube temperature of ELSD was 100℃ with carrier gas flow rate of 2.9 mL/min. The contents of spinosin ......