李光超 张倩 武婧

    [摘要] 目的 分析STAT1基因敲除小鼠免疫相关因子表达差异，观察STAT1基因敲除小鼠感染EV71病毒后的症状表现及病毒载量，为EV71抗病毒治疗提供数据基础。 方法 LPS刺激STAT1基因敲除小鼠后，利用微珠免疫分析(CBA)方法对血清免疫相关因子定量分析；EV71感染STAT1基因敲除小鼠后，观察症状及病理表现，利用实时荧光定量检测对肌肉病毒RNA定量分析。 结果 LPS刺激后，STAT1基因敲除小鼠血清多种细胞因子分泌水平下降(P < 0.01)；EV71感染后，STAT1基因敲除小鼠病理损伤加重，肌肉中病毒载量升高(P < 0.01)，与野生型小鼠相比表现出更严重的症状与更高的死亡率。 结论 STAT1在抗EV71感染的免疫过程中扮演重要角色，提示STAT1基因敲除可以提高小鼠对EV71病毒的敏感性。

    [关键词] 病毒感染；EV71；STAT1；炎症因子

    [中图分类号] R373 [文献标识码] A [文章编号] 1673-7210(2017)05(c)-0014-05

    [Abstract] Objective To analyze the differential expression of immune-associated factors in STAT1 knockout mice， and observe the symptoms and viral load after EV71 virus infection， in order to provide a data base for anti-EV71 therapy. Methods Cytometric bead assay (CBA) was used to quantitative analysis the levels of serum immune-associated factors in STAT1 knockout mice after LPS-induced treatment. The symptom and tissue pathological features were observed and the level of muscle virus RNA was quantitated by real-time PCR in STAT1 knockout mice after EV71 virus infection. Results The levels of multi- immune-associated factors were decreased in STAT1 knockout mice after LPS stimulating (P < 0.01) ......