康肖++扈文海++裴世深++王清和++刘璞++李士学

    [摘要] 目的 研究斑蝥酸钠维生素B6诱导MG-63细胞凋亡的作用机制。 方法 应用不同浓度的斑蝥酸钠维生素B6作用于MG-63细胞，用MTT比色法检测药物对细胞的增殖抑制作用；反转录聚合酶链反应(RT-PCR)检测存活素信使核糖核酸的表达。 结果 斑蝥酸钠维生素B6在抑制MG-63细胞增殖方面有显著作用，其作用具有时间和剂量依赖性，抑制率可高达(68.56±5.21)%。RT-PCR显示可下调survivin基因表达。 结论 斑蝥酸钠维生素B6抑制MG-63细胞增殖效果明显，其机制可能与下调survivin基因的表达有关。

    [关键词] 斑蝥酸钠维生素B6；MG-63；细胞凋亡；存活素

    [中图分类号] R285.5 [文献标识码] A [文章编号] 1673-7210(2017)06(a)-0016-04

    Effects of Sodium Cantharidate and vitamin B6 on apoptosis and survivin expression in human osteosarcoma cells

    KANG Xiao1 HU Wenhai2 PEI Shishen1 WANG Qinghe1 LIU Pu1 LI Shixue1

    1.The Third Department of Orthopedics， Hengshui Fourth People's Hospital， Hebei Province， Hengshui 053000， China； 2.Department of Bone Oncology， the Third Hospital of Hebei Medical University， Hebei Province， Shijiazhuang 050051， China

    [Abstract] Objective To study the mechanism of Sodium Cantharidate and vitamin B6 inducing apoptosis of MG-63 cells. Methods The different concentrations of Sodium Cantharidinate and vitamin B6 were applied in MG-63 cells， the anti-proliferative effect of drug for cells was detected by MTT colorimetry； reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of survivin messenger RNA. Results Sodium Cantharidate and vitamin B6 could inhibit the proliferation of MG-63 cells in a time and dose-dependent manner ......