孙丹++++++张艳++++++孙强++++++彭玉勃++++++王磊

    [摘要] 目的 研究民族药刺齿凤尾蕨抑制人源结肠癌细胞活性成分。 方法 采用95%乙醇提取药材，聚酰胺色谱、葡聚糖凝胶色谱以及制备型高效液相色谱等技术获取化合物，磁共振波谱技术(NMR)对得到的化合物进行结构鉴定。磺酰罗丹明B比色法(SRB)对化合物进行体外抗肿瘤活性筛选。 结果 从刺齿凤尾蕨中分离得到5个二萜类化合物，分别鉴定为geopyxin A(1)，geopyxin C(2)，ent-11β-acetoxykaur-16-en-18-ol(3)，7β，16α，17-trihydroxy-ent-kauran-19-oic acid(4)和neolaxiflorin K(5)。活性筛选表明化合物1，2和5具有显著的抑制人源结肠癌细胞活性，IC50值分别为1.20、2.45和3.26 μmol/L。 结论 化合物1～5首次从刺齿凤尾蕨中分离得到。

    [关键词] 刺齿凤尾蕨；化学成分；体外抗肿瘤活性

    [中图分类号] R927 [文献标识码] A [文章编号] 1673-7210(2017)06(a)-0020-04

    Study on chemical constituents and their inhibition of anthropogenic colon cancer cells bioactivity from ethnic medicine Pteris dispar

    SUN Dan ZHANG Yan SUN Qiang PENG Yubo WANG Lei

    Jiamusi College， Heilongjiang University of Chinese Medicine， Heilongjiang Province， Jiamusi 154007， China

    [Abstract] Objective To study the inhibition of anthropogenic colon cancer cells activity constituents of Pteris dispar. Methods The medicinal materials were extracted by 95% ETOH. Compounds were obtained by kinds of chromatographies including polyamide， Sephadex LH-20 and pre-HPLC and the structures identified by NMR. In vitro antitumor activity was tested by SRB methods. Results 5 compounds were obtained from the plant and their structures were established as geopyxin A (1) ......