李建梅 周凡 希尔艾力·吐尔逊

    [摘要] 目的 采用反相高效液相色谱法，建立同时测定补骨脂提取物中补骨脂素、异补骨脂素和补骨脂酚含量的方法。 方法 采用Epic C18(250 mm×4.6 mm，5 μm)色谱柱；流动相：甲醇-水，梯度洗脱；流速：1.0 mL/min；柱温：30℃；检测波长250 nm。 结果 该法测定补骨脂素、异补骨脂素和补骨脂酚分别在0.0563～0.3379、0.0523～0.3139、0.1280～0.7680 μg范围内线性关系良好，相关系数均为0.9999。平均加样回收率分别为100.66%(n=6)、RSD=1.77%，100.43%(n=6)、RSD=1.45%和99.41%(n=6)、RSD=1.63%。 结论 本方法简便、准确、灵敏、重复性好，可作为补骨脂提取物中补骨脂素、异补骨脂素和补骨脂酚的含量控制方法。

    [关键词] 补骨脂；补骨脂素；异补骨脂素；补骨脂酚；反相高效液相色谱

    [中图分类号] R969 [文献标识码] A [文章编号] 1673-7210(2017)09(c)-0012-04

    [Abstract] Objective To determine psoralen， isopsoralen and bakuchiol from the extract of Psoralea corylifolia by RP-HPLC. Methods The contents of paoralen， isopsoralen and bakuchiol were determined on the Epic C18 (250 mm×4.6 mm， 5 μm) column， with methanol： water in gradient elution manner. The flow rate was 1.0 mL/min， the column temperature was maintained at 30℃， and the detection wavelength was set up at 250 nm. Results The linear ranges of paoralen， isopsoralen and bakuchiol were 0.0563-0.3379， 0.0523-0.3139 and 0.1280-0.7680 μg (r=0.9999). The average recovery of paoralen， isopsoralen and bakuchiol were 100.66% ......