李瑞雪 吴飞 张继全

    [摘要] 目的 以一测多评法建立香加皮提取物中7种C21甾体皂苷成分的高效液相色谱分析方法。 方法 采用Capcell Pak C18色谱柱，流动相乙腈-0.2%甲酸水溶液梯度洗脱，检测波长263 nm，以杠柳苷A(PSA)为内参物，建立其与杠柳苷K(PSK)、杠柳苷R(WJ53)、杠柳苷E(WJ521)、杠柳苷D(PSD)、杠柳苷Q(WJ33)和杠柳苷O(WJ32)的相对校正因子，并进行含量计算，实现一测多评，并将一测多评法测定结果与外标法测定结果对比，验证一测多评法的准确性。 结果 PSA与PSK、WJ53、WJ521、PSD、WJ33和WJ32的相对校正因子分别为0.867、0.867、0.847、0.994、0.922和1.029。采用校正因子计算的含量值与外标法实测值之间没有显著性差异。 结论 该方法准确可靠，简便可行，可作为香加皮提取物中间体多指标成分测定的质量控制方法。

    [关键词] 香加皮提取物；C21甾体皂苷；一测多评法；相对校正因子；杠柳苷A；高效液相色谱法；外标法

    [中图分类号] R284.1 [文献标识码] A [文章编号] 1673-7210(2017)09(c)-0021-04

    [Abstract] Objective To establish a high performance liquid chromatography method for quantitative analysis of seven C21 steroidal saponins in Cortex periplocae extract by quantitative analysis of multi-components by single-marker. Methods The high performance liquid chromatography analysis was performed on a Capcell Pak C18 column with the detection wavelength at 263 nm. The mobile phase was water containing 0.2% formic acid and acetonitrile in the gradient mode. Periploside C (PSA) was used as the internal marker to establish relative correction factors between PSA and periploside K (PSK) ......