徐文峰++++++徐硕++++++何笑荣++++++姜文清++++++吴学军++++++邝咏梅++++++金鹏飞

    [摘要] 目的 应用高效液相色谱法(HPLC)建立天然维生素E软胶囊的含量测定方法。 方法 采用Alltima C18色谱柱(150 mm × 4.6 mm，5 μm)，以甲醇为流动相，流速1.0 mL/min，检测波长285 nm。 结果 在本方法中，维生素E的线性关系良好，线性相关系数为0.9999；专属性强，精密度和稳定性良好，相对标准偏差(RSD)分别为0.17%和0.87%；平均加样回收率(n = 6)为100.8%，加样回收率良好。含量测定结果显示3个批号样品中维生素E的含量分别为标示量的101.2%、100.8%、102.7%。 结论 该方法准确、简单、快速，可用于天然维生素E软胶囊的含量测定。

    [关键词] 天然维生素E软胶囊；维生素E；高效液相色谱法；含量测定

    [中图分类号] R927 [文献标识码] A [文章编号] 1673-7210(2017)10(c)-0136-04

    Determination of narural vitamin E in Natural Vitamin E Soft Capsules

    XU Wenfeng XU Shuo HE Xiaorong JIANG Wenqing WU Xuejun KUANG Yongmei JIN Pengfei

    Department of Pharmacy， Beijing Hospital National Center of Gerontology， Beijing 100730， China

    [Abstract] Objective To establish a high performance liquid chromatography (HPLC) method for the determination of narural vitamin E in Natural Vitamin E Soft Capsules. Methods An Alltima C18 column (150 mm× 4.6 mm， 5 μm) was used for the separation， with methanol as the mobile phase at the flow rate of 1.0 mL/min. The detection wavelength was 285 nm. Results The method showed good linearity with correlation coefficient of 0.9999 for vitamin E. The assay method showed good specificity. The precision and stability were satisfactory with the relative standard deviations (RSD) of 0.17% and 0.87% respectively. The average spiked recovery was 100.8% (n = 6) ......