美热古丽·依米提 窦勤 李治建 斯拉甫·艾白

    [摘要] 目的 研究没食子提取物体内外抗炎作用。 方法 体内以二甲苯致小鼠耳肿胀、角叉菜胶致大鼠足趾肿胀实验观察其抗炎作用；体外观察没食子提取物对RAW264.7细胞生长曲线和活力以及吞噬中性红能力的影响；采用酶联免疫吸附测定法(ELISA)检测没食子提取物对脂多糖(LPS)刺激的RAW264.7细胞分泌肿瘤坏死因子α(TNF-α)的影响。 结果 与模型组比较，没食子提取物能显著减轻小鼠的耳肿胀度和足趾肿胀(P < 0.05或P < 0.01)；20、40 μg/mL没食子提取物能活化RAW264.7巨噬细胞，促进RAW264.7细胞增殖，并能增强其吞噬功能；25、50 μg/mL没食子提取物对LPS诱导的RAW264.7细胞分泌TNF-α有明显的抑制作用(P < 0.01)。 结论 没食子提取物具有一定的抗炎作用，可能與抑制促炎介质TNF-α的分泌有关。

    [关键词] 没食子提取物；抗炎作用；RAW264.7细胞；肿瘤坏死因子α

    [中图分类号] R285.5 [文献标识码] A [文章编号] 1673-7210(2018)02(c)-0008-06

    [Abstract] Objective To study the anti-inflammatory effects of Turkish galls extract in vivo and in vitro. Methods The anti-inflammatory effects in vivo were observed through the experiments of mice ear swelling induced by xylene and rats toe swelling induced by carrageenan； the effects of Turkish galls extract for the growth curve and vitality of RAW264.7 cells and phagocytosis of neutral red were observed in vitro； the effects of Turkish galls extract for the secretion of tumor necrosis factor α (TNF-α) in RAW264.7 cells stimulated by lipopolysaccharide (LPS) were detected by enzyme linked immunosorbent assay (ELISA). Results Compared with model group ......