陈蔚 鞠婧 王浩

    [摘要] 目的 探討黄芪多糖对糖尿病心肌氧化应激的影响。 方法 将16只6～8周龄的雄性健康转基因SOD2+/-KO小鼠(C57BL/6J背景)按照随机数字表法随机分为转基因组及黄芪多糖转基因组(n = 8)；将24只正常对照小鼠分为对照组、糖尿病组及黄芪多糖糖尿病组(n = 8)。糖尿病组和黄芪多糖糖尿病组采用链脲佐菌素腹腔注射法制作糖尿病动物模型，糖尿病模型建成功后，给予黄芪多糖糖尿病组和黄芪多糖转基因组小鼠黄芪多糖注射液治疗16周，其余组则给予等量生理盐水灌胃。采用超声心动图检测心脏功能，HE染色观察心肌组织结构，超声电镜观察心肌超微结构，免疫组化法检测心肌细胞增殖活性，TUNEL法检测心肌细胞凋亡，荧光定量法检测心肌活性氧(ROS)含量，Western blot法检测心肌SOD2蛋白含量，SODⅡ试剂盒检测心肌SOD2酶活性。 结果 与对照组比较，糖尿病组和转基因组LVSP、±LVdp/dt下降，LVEDP升高，心肌组织和超微结构存在严重损伤，细胞凋亡和坏死程度增加，SOD2活性被显著抑制，而黄芪多糖治疗组小鼠以上指标均得到改善(P < 0.05)。 结论 黄芪多糖能抑制糖尿病心肌的氧化应激损伤。

    [关键词] 黄芪多糖；糖尿病；心肌病；氧化应激；SOD2

    [中图分类号] R5871.1 [文献标识码] A [文章编号] 1673-7210(2018)03(c)-0004-04

    [Abstract] Objective To investigate the effect of Astragalus polysaccharides for myocardial oxidative stress of diabetes mellitus. Methods Sixteen male healthy transgenic SOD2+/-KO mice aged 6-8 weeks old (C57BL/6J) were randomly divided into transgenic group and Astragalus polysaccharides transgenic group by random number table method (n = 8). The 24 normal control mice were divided into control group， diabetes mellitus group and Astragalus polysaccharides diabetes mellitus group (n = 8). Single intraperitoneal injection of Streptozotocin was utilized to establish mice model with diabetes mellitus in diabetes mellitus group and Astragalus polysaccharides diabetes mellitus group ......