李书坛 黄纯兰

    [摘要] 目的 研究BMP-2联合人骨髓间充质干细胞(MSC)造血干细胞(HSC)体外增殖的影响。 方法 培养MSC至第三代，磁珠分选仪分选HSC，并用流式细胞术鉴定MSC与HSC。将获得的MSC与HSC利用Transwell非接触共培养，100 ng/mL的骨形态发生蛋白(BMP-2)干预，即实验分四组：HSC组、HSC+BMP2组、HSC+MSC组、HSC+MSC+BMP2组。培养3 d后比较不同培养条件下HSC计数、RNA浓度的不同，并通过实时荧光定量PCR法及免疫荧光方法检测HSC的Ki67的mRNA及蛋白的表达。 结果 HSC的计数、总RNA含量、Ki67的表达在HSC+BMP2组高于HSC组、HSC+MSC组高于HSC组、HSC+MSC+BMP2组高于HSC+MSC组(P < 0.05)。 结论 MSC协同BMP-2蛋白可促进HSC的增殖。

    [关键词] 造血干细胞；间充质干细胞；BMP-2；增殖

    [中图分类号] R331.2 [文献标识码] A [文章编号] 1673-7210(2018)04(c)-0004-05

    The effect of bone marrow mesenchymal stem cell synergistic BMP-2 in the proliferation of hematopoietic stem cell

    LI Shutan HUANG Chunlan

    Department of Hematology， the Affiliated Hospital of Southwestern Medical University， Sichuan Province， Luzhou 646000， China

    [Abstract] Objective To explore the proliferation effect of bone morphogenetic protein (BMP)-2 on hematopoietic stem cell (HSC) cocultured with mesenchymal stem cell (MSC). Methods MSCs were expanded to the third passage (P3) in the plastic dish； CD34+ cells were sorted by MACS Miltenyi Biotec. MSCs(P3) and HSCs were identified by FCM separately ......