李华杰 徐雄峰 邱波

    [摘要] 间充质干细胞(MSCs)来源广泛并具有多向分化潜能，被视为组织修复重建领域的新希望。但近年的研究表明，传统的贴壁培养模式改变了细胞生长的原始环境，会导致MSCs自主分化、干性衰退等诸多问题，移植体内后治疗效果不佳。研究证据表明，相较于传统的贴壁培养，3D-球体培养方式对MSCs的生物活性具有显著促进作用。壳聚糖薄膜(CM)培养是一种新型生物膜诱导MSCs成球方法，本文主要从MSCs形态学、生物功能的变化及其潜在机制方面对MSCs的CM成球培养模式进行综述，并探讨其在MSCs临床应用方面的潜力。

    [关键词] 间充质干细胞；3D-球体；壳聚糖薄膜；干性

    [中图分类号] R318 [文献标识码] A [文章编号] 1673-7210(2018)05(b)-0021-05

    Research progress and clinical application prospect in 3D-sphere culture of mesenchymal stem cell with chitosan membrane

    LI Huajie XU Xiongfeng QIU Bo

    Department of Orthopedics， Renmin Hospital of Wuhan University， Hubei Province， Wuhan 430060， China

    [Abstract] Mesenchymal stem cells (MSCs) with a wide range of sources and multipotential differentiation are thought as a new hope in the field of tissue repair and reconstruction. However， recent research shows that traditional adherent culture changes the initial growing environment of MSCs， leading to self-differentiation， stemness recession and other problems， which brings a negative effect to the treatment after in vivo transplantation. And the evidence suggests that ......