赵瑞 姚黎清

    [摘要] 目的 观察人类ApoE3基因过表达对大鼠肾上腺嗜铬细胞瘤细胞PC12凋亡的影响。 方法 将PC12转染含人ApoE3基因的pEGFP质粒及空载体pEGFP质粒，经持续筛选建立起稳定表达人ApoE3基因和质粒pEGFP的PC12的细胞系。将细胞分成4组：正常对照组(用正常培养基培养)、H2O2组(用400 μmol/L H2O2诱导4 h)、H2O2+空质粒组(细胞转染pEGFP经筛选后用H2O2诱导4 h)、H2O2+ApoE3组(细胞转染ApoE3经筛选后用H2O2诱导4 h)。通过CCK-8检测细胞存活率，Hochest33258检测细胞凋亡。 结果 与正常对照组比较，H2O2组、H2O2+空质粒组和H2O2+ApoE3组细胞存活率下降(P 0.05)；H2O2+ApoE3组细胞存活率明显高于H2O2组、H2O2+空质粒组(P < 0.05)，H2O2组+ApoE3组细胞凋亡较H2O2组和H2O2+空质粒组明显减少。 结论 ApoE3基因高表达对H2O2所致的PC12细胞氧化损伤有很好的保护作用。

    [关键词] PC12细胞；载脂蛋白E3基因；过氧化氢；凋亡

    [中图分类号] R744 [文献标识码] A [文章编号] 1673-7210(2018)06(b)-0004-04

    [Abstract] Objective To observe the effect of human ApoE3 gene high expression on the apoptosis of rat adrenal pheochromocytoma PC12 cells. Methods The pEGFP plasmid containing human ApoE3 gene and pEGFP plasmid were transfected with PC12 cells， and PC12 cell line was established that could stably express human ApoE3 gene and pEGFP plasmid through continuous screening. PC12 cells were allocated into 4 groups： normal control group， H2O2 group (cultured with 400 μmol/L H2O2 for 4 hours)， liposome group (pEGFP transfection and H2O2 culture) and ApoE3 group (ApoE3 transfection and H2O2 culture). Cell survival was tested by CCK-8 method ......