孙逊沙 吴洁莹 陈劲松 陆琰 喻秋霞 李茹 王丹 李焱 吴韶清

    [摘要] 目的 探讨环状RNA在人胎盘绒毛膜间充质干细胞低氧预处理和常氧培养中表达谱的差异。 方法 利用芯片技术检测3例低氧预处理人胎盘绒毛膜间充质干细胞及其相应对照的常氧培养细胞的环状RNA表达，分析两者差异表达的环状RNA及其结合miRNA。 结果 在检测的13 617条环状RNA中，有分析结果的环状RNA 12 114条，低氧和常氧培养人胎盘间充质干细胞差异表达环状RNA共102条，其中低氧中表达上调的85条，下调的17条(倍数变化>1.5倍且P < 0.05)，而表达差异倍数变化大于2倍以上的环状RNA有27条，且均为上调表达。每个环状RNA预测到5个结合miRNA。 结论 低氧预处理使得人胎盘绒毛膜间充质干细胞的环状RNA表达谱发生了显著变化，这些环状RNA可能和低氧预处理有关。

    [关键词] 低氧；人胎盘绒毛膜间充质干细胞；环状RNA；表达谱

    [中图分类号] R394.2 [文献标识码] A [文章编号] 1673-7210(2018)06(c)-0009-03

    [Abstract] Objective To analyze the expression profile variation of circular RNA (circRNA) between hypoxia preconditioned human placenta chorionic mesenchymal stem cells (H-hpcMSCs) and normoxic preconditioned hpcMSCs (N-hpcMSCs). Methods Human circRNA microarray was performed to detect the difference of circRNA expression between 3 paired specimens of H-hpcMSCs and its N-hpcMSCs. The circRNA differental expression and the predicted miRNA of differentially expressed circRNA were analyzed. Results Of the 13 617 circRNAs， 12 114 circRNAs with analytical results. In H-hpcMSCs ......