肖婷焱 段无暇 许紫薇 周寿红 曾斌

    [摘要] 目的 观察转录激活因子4(ATF4)过表达对人肝癌HepG2细胞凋亡的影响，并探讨其机制。 方法 构建ATF4表达质粒，采用脂质体瞬时转染HepG2细胞。实验分为空白对照组、空质粒组和ATF4质粒组。采用流式细胞术检测细胞凋亡率，检测HepG2细胞培养液中乳酸含量，Western blot检测Warburg效应蛋白M2型丙酮酸激酶(PKM2)的表达。 结果 与空质粒组比较，ATF4质粒组HepG2细胞凋亡率显著增加，细胞培养液中乳酸含量显著降低，HepG2细胞中PKM2蛋白的表达显著下调(均P < 0.05)。 结论 过表达ATF4促进HepG2细胞的凋亡，其机制可能与抑制Warburg效应有关。

    [关键词] 肝细胞性肝癌；细胞凋亡；转录激活因子4；Warburg效应；M2型丙酮酸激酶

    [中图分類号] R34 [文献标识码] A [文章编号] 1673-7210(2018)07(a)-0012-05

    Effect and mechanism of overexpression of ATF4 on the apoptosis of HepG2 cells

    XIAO Tingyan1 DUAN Wuxia1 XU Ziwei1 ZHOU Shouhong2 ZENG Bin1

    1.Department of Gastroenterology， the First Affiliated Hospital of University of South China， Hu'nan Province， Hengyang 421001， China； 2.Teaching and Research Office of Physiology， Medical College， University of South China， Hu'nan Province， Hengyang 421001， China

    [Abstract] Objective To observe the effect of overexpression of activating transcription factor 4 (ATF4) on the apoptosis of HepG2 cells and explore the mechanism. Methods ATF4 expression plasmid was built. Hepatocellular carcinoma line HepG2 cells were transfected with ATF4 expression plasmid through liposome. The plasmids were divided into blank control group ......