潘瑞 杜业亮 牟伟男

    [摘要] 目的 探讨蛛网膜下腔出血(SAH)并发脑心综合征(CCS)大鼠肾素-血管紧张素的变化。 方法 枕大池注入自体动脉血法复制SAH模型，健康雄性Wistar大鼠75只，6周龄，体重150～200 g，所有大鼠均与动力实验室系统连接(Power Lab系统)检测心电图，排除异常心电图者。将动物随機分为手术组(50只)、假手术组(25只)。手术组根据术后心电图不同分为SAH组、CCS组，每组各25只(不足者同条件新鼠补入)，各组大鼠按取材时段不同随机分为1、3、5、7、14 d等5个亚组，每组5只。造模后用Power Lab系统检测心电图，分别于1、3、5、7、14 d再次检测心电图，用酶联免疫吸附测定(ELISA)法检测血浆中肾素活性(PRA)及血管紧张素Ⅱ(AngⅡ)的含量。 结果 造模成功后，假手术组心电图未见明显变化，手术组术后2 h CCS病发率最高。1 d时SAH组、CCS组PRA、AngⅡ含量均高于假手术组(P 0.05)。 结论 SAH并发CCS的PRA、AngⅡ呈逐渐上升达高峰后逐渐下降趋于稳定的变化，提示PRA、AngⅡ在SAH并发CCS的发生与发展中起到了一定的作用。

    [关键词] 脑心综合征；蛛网膜下腔出血；肾素活性；血管紧张素Ⅱ

    [中图分类号] R743.3 [文献标识码] A [文章编号] 1673-7210(2018)08(c)-0018-04

    [Abstract] Objective To investigate the changes of renin-angiotensin in rats with subarachnoid hemorrhage (SAH) complicated by cerebral-cardiac syndrome (CCS). Methods The occipital large pool was injected with autologous arterial blood to replicate the SAH model. Seventy-five healthy male Wistar rats were 6 weeks old and weighed 150-200 g. All rats were connected to a dynamic laboratory system (Power Lab system) to detect ECG， excluding abnormal ECG. The rats were randomly divided into surgery group (50 rats) and sham group (25 rats). The surgical group was divided into SAH group and CCS group according to different electrocardiograms after surgery. Each group had 25 animals (insufficient ones were recruited in the same condition as new rat). Different time periods were randomly divided into five subgroups： 1 ......