邓思珊 刘洪旭 马丽红

    [摘要] 目的 定性、定量分析三葉青叶黄酮类化学成分。 方法 采用超高效液相色谱-串联质谱(UPLC-MS/MS)对三叶青叶化学成分进行鉴定，色谱柱为C18 Luna Omega(2.1 mm×100 mm，1.6 μm)；流动相：乙腈-水(50∶50，V/V)；流速0.4 mL/min；柱温35℃；进样量5 μL；全扫描模式扫描。采用高效液相色谱法(HPLC)同时测定三叶青叶中5种碳苷黄酮的含量。色谱柱为Ultimate XB C18(250 mm×4.6 mm，5 μm)；流动相为乙腈-0.2% H3PO4(10∶90，V/V)；流速为1 mL/min；检测波长为350 nm；室温；进样量20 μL。 结果 三叶青叶中共鉴定出6个化学成分，其中5个黄酮成分的保留时间、光谱图和质谱碎片离子信息与对照品一致，分别为异荭草素、荭草素、牡荆素鼠李糖苷、牡荆素、异牡荆素。HPLC测定8个不同产地来源的样品中上述5种成分含量，各成分线性关系良好(r > 0.9997)，加样回收率在95.0%～105.0%之间。 结论 三叶青叶子中主要含2类碳苷黄酮，即荭草素类和牡荆素类；本研究建立的方法可定性、定量分析分析三叶青叶中主要化学成分，可用于控制药材质量。

    [关键词] 三叶青；黄酮类；超高效液相色谱-串联质谱；高效液相色谱

    [中图分类号] R284.1 [文献标识码] A [文章编号] 1673-7210(2018)11(c)-0080-06

    [Abstract] Objective Qualitative and quantitative analysis of the chemical constituents of flavonoids in the leaves of Tetrastigma hemsleyanum. Methods The chemical constituents in leaves of Tetrastigma hemsleyanum were identified by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The column was C18 Luna Omega (2.1 mm × 100 mm， 1.6 μm)； the mobile phase was acetonitrile-water (50∶50， V/V)； the flow rate was 0.4 mL/min； the column temperature was 35°C； the injection volume was 5 μL； full scan mode. The contents of five carbon glycosides in the leaves of Tetrastigma hemsleyanum were determined at the same time by high performance liquid chromatography (HPLC). The column was Ultimate XB C18 (250 mm×4.6 mm ......