陈宇 郑新 陈立波

    [摘要] 目的 建立快速测定母乳中拉贝洛尔含量的超高效液相色谱串联质谱法(UPLC-MS/MS)。 方法 母乳样品经甲醇-乙腈混合溶液沉淀蛋白，稀释后离心取上清液进样。色谱柱为Thermo Scientific Syncronis C18(100 mm×2.1 mm，1.7 μm)，初始流动相为0.2%甲酸溶液-乙腈(80∶20，V/V)，流速为0.2 mL/min，梯度洗脱，AJS ESI正离子模式电离，多反应离子监测模式(MRM)检测，外标法定量。 结果 母乳中拉贝洛尔在1.042～104.2 ng/mL范围内线性良好(r = 0.9988)，定量下限为1.042 ng/mL，准确度为92.0%～104.8%，批内、批间RSD均小于15%，提取回收率为82.2%～92.4%。 结论 本方法前处理简单、准确，专属性强、灵敏度高，可用于拉贝洛尔的乳药浓度监测。

    [关键词] 拉贝洛尔；超高效液相色谱串联质谱法；乳药浓度；药物监测

    [中图分类号] RTS218；O657.63 [文献标识码] A [文章编号] 1673-7210(2018)11(c)-0085-04

    [Abstract] Objective To establish an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for rapid determination of labetalol in breast milk. Methods The breast milk sample was precipitated by a methanol-acetonitrile mixed solution， and after centrifugation， the supernatant was centrifuged for injection. The column was Thermo Scientific Syncronis C18 (100 mm × 2.1 mm， 1.7 μm)， the initial mobile phase was 0.2% formic acid solution-acetonitrile (80∶20， V/V)， the flow rate was 0.2 mL/min， utilizing a gradient elution program. The MS/MS detection was performed under AJS ESI+ in MRM mode ......