董文凤 杜向东

    [摘要] 目的 研究長期服用奥氮平对大鼠糖原代谢及糖转运蛋白表达的影响，探讨奥氮平影响糖代谢的可能机制。 方法 将12只健康雄性SPF级SD大鼠按照随机数字表法分为对照组(n = 6)和药物组(n = 6)，药物组大鼠每天以奥氮平混悬液2.1 mg/kg体重灌胃，对照组大鼠每天以等体积蒸馏水灌胃，持续63 d。定期监测大鼠的摄食量、体重变化、空腹血糖(FBG)和空腹胰岛素(FINS)，计算胰岛素敏感指数(ISI)和胰岛素抵抗稳态评价模型指数(HOMA-IR)。给药63 d后采集两组大鼠的肝脏、肌肉和脂肪组织，检测肝脏和肌肉组织中糖原含量和糖原合酶(GS)活性，Western blot法检测肝脏和肌肉组织中GS以及肝脏、肌肉和脂肪组织中葡萄糖转运体(GLUT)的蛋白表达，PCR法检测肝脏、肌肉和脂肪组织中GLUT的mRNA表达。 结果 与对照组比较，长期给予奥氮平后药物组大鼠摄食量和体重增加，FBG和FINS升高，糖原含量减少，GS活性提高但蛋白表达不变;GLUT蛋白表达和mRNA表达均降低，差异有高度统计学意义(P < 0.01)。 结论 在奥氮平长期诱导下，机体糖原分解增加，糖转运能力下降，产生胰岛素抵抗，这可能是长期服用奥氮平后糖代谢异常的机制之一。

    [关键词] 奥氮平;血糖;糖原;葡萄糖转运体

    [中图分类号] R749.3? ? ? ? ? [文献标识码] A? ? ? ? ? [文章编号] 1673-7210(2019)01(c)-0011-05

    [Abstract] Objective To study the effects of subchronic Olanzapine treatment on the metabolism of glycogen and expression of glucose transporter in rats and to investigate the possible mechanism of Olanzapine′s effects on glycometabolism. Methods Twelve healthy male SPF grade Sprague-Dawley (SD) rats were divided into control group (n = 6) and drug group (n = 6) according to random number table method. Rats in drug group were given 2.1 mg/kg Olanzapine suspension per day according to body weight by gavage and rats in control group were given the same volume of distilled water by gavage for 63 days. The food intake ......