许聪聪 林鹭 梁惠芬 安红梅 胡兵 张彤 浦益琼

    [摘要] 目的 建立蛇莓药材中对羟基桂皮酸的高效液相色谱测定法。 方法 采用Ultimate XB-C18(250 mm×4.6 mm，5 μm)色谱柱，乙腈-0.2%醋酸(14∶86)为流动相，等度洗脱，流速为1.0 mL/min，检测波长为300 nm，柱温30℃。结果 对羟基桂皮酸浓度在0.413～26.450 μg/mL范围内与峰面积呈良好的线性关系，线性回归方程为Y=72.592X +1.1304(r = 0.9999)，平均加样回收率为103.52%，RSD为2.61%。3批蛇莓中对羟基桂皮酸的含量分别为0.11、0.10、0.05 mg/g。 结论 该方法方便快速，其分离效果好，灵敏度高，重复性好，可用于蛇莓药材的质量控制。

    [关键词] 蛇莓;对羟基桂皮酸;高效液相色谱法;含量测定

    [中图分类号] R284.1? ? ? ? ? [文献标识码] A? ? ? ? ? [文章编号] 1673-7210(2019)08(a)-0033-05

    [Abstract] Objective To establish a method for the determination of p-hydroxycinnamic in Duchesnea indica (Andr.) by HPLC. Methods Ultimate XB-C18(250 mm×4.6 mm， 5 μm) column was used as the column， acetonitrile-0.2% acetic acid solution (14∶86) was used as the mobile phase of the isocratic elution， with the detection wavelength of 300 nm and the flow rate of 1.0 mL/min. The column temperature maintained at 30℃. Results The concentration of hydroxycinnamic acid in the range of 0.413-26.450 μg/mL showed a good linear relationship with the peak area ......