张雪 陈艳焦 程觅

    [摘要] 血清蛋白生物标志物是标记疾病过程中组织、器官或细胞功能变化的重要分子指标，在医学诊断和治疗中具有重要应用价值。酶促化学发光免疫分析、多重荧光免疫分析、电化学发光免疫分析等技术的发展为不同的血清蛋白生物标志物提供了多样的检测手段。本文对目前常用血清蛋白生物标志物检测方法的技术特点、应用研究和优势与不足进行了分析比较，为研究者选择合适的方法提供参考。

    [关键词] 血清;蛋白标志物;免疫法;检测技术

    [中图分类号] R446 ? ? ? ? ?[文献标识码] A ? ? ? ? ?[文章编号] 1673-7210(2019)12(c)-0024-06

    Advance in the immunological detection technologies research of serum protein biomarkers

    ZHANG Xue ? CHEN Yanjiao ? CHENG Mi ? MAO Junxia ? SHI Yanglin ? YANG Yongqing ? XU Yudong

    Shanghai University of Traditional Chinese Medicine， Shanghai Research Institute of Acupuncture & Meridian， Shanghai ? 200030， China

    [Abstract] Serum protein biomarkers are important molecular markers for labeling changes in tissues， organs and cell functions during disease， which have important application value in disease diagnosis and treatment. The development of immunological assays such as enzymatic chemiluminescence immunoassays， multiplex fluorescence immunoassays， and electrochemiluminescence immunoassays provides various methods for serum protein biomarkers detection. In this review ......