李慧 杨会 白云绮

    [摘要] 目的 筛选四妙勇安汤具有过氧化物酶体增殖物激活受体γ(PPAR-γ)激活作用的活性部位，并对其化学成分进行分析，探讨提取物的活性成分和抗炎作用。 方法 采用醇水提取、树脂分离获得四妙勇安汤的8个提取物，以PPAR-γ为靶点，于细胞水平筛选四妙勇安汤提取物的活性部位，对有活性趋势的提取物绘制剂量依赖性曲线，计算EC50值以及相对活性，并采用液相色谱-串联质谱法对活性部位的化学成分进行分析鉴定。 结果 PPAR-γ激动模型中，提取物CS04及CS05具有较强的PPAR-γ激活作用。对活性部位CS04和CS05进行化学成分鉴定，共鉴别出31个成分。 结论 四妙勇安汤提取物CS04和CS05具有较强激活PPAR-γ作用，这一作用可能与其抑制动脉粥样硬化炎症有关。

    [关键词] 四妙勇安汤提取物;过氧化物酶体增殖物激活受体γ激动剂;激活作用;化学成分;液质联用技术

    [中图分类号] R286.0 ? ? ? ? ?[文献标识码] A ? ? ? ? ?[文章编号] 1673-7210(2020)06(a)-0028-06

    [Abstract] Objective To screen the active parts of Simiao Yong′an Decoction with peroxisome proliferators-activated receptor γ (PPAR-γ) activation and analyze its chemical components， and to explore the active components and anti-inflammatory effects of the extract. Methods Eight alcohol extracts of Simiao Yong′an Decoction were obtained by alcohol extraction and resin separation. The PPAR-γ was used as the target to screen the active parts of the extract of Simiao Yong′an Decoction at the cell level. A dose-dependent curve was drawn for the extracts with active tendency. EC50 value and relative activity value were calculated ......