[摘要] 目的 优化PCR程序,尝试用降落PCR扩增人类常染色体STR D15S128。方法 用普通PCR和降落PCR扩增人类常染色体STR D15S128。结果 普通PCR扩增产物有非特异带,降落PCR无非特异带。结论 降落PCR简化了普通PCR中摸索最适退火温度的过程,可以一步找到人类常染色体STR D15S128的退火温度,是一种高效率的PCR方法。

    [关键词] D15S128;降落PCR;退火温度;PCR特异性

    [中图分类号] Q781 [文献标识码] A[文章编号] 1673-9701(2010)02-38-02

    Touchdown PCR for Amplification of Human Autosomal STR D15S128

    ZHANG YanpingGUO DaweiMA Lili

    College of Forensic Medicine of Shanxi Medical University,Taiyuan 030001,China

    [Abstract] Objective To optimize PCR program and try to amplify human autosomal STR D15S128 by touchdown PCR(TD-PCR).Methods We used both common PCR and TD-PCR to amplify human autosomal STR D15S128. Results Ordinary amplification showed non-specificity band and TD-PCR showed no non-specificity band. Conclusion Compared with ordinary PCR,TD-PCR is simple and effective.

    [Key words] D15S128;TD-PCR;Annealing temperature;PCR specificity

    PCR(polymerase chain reaction)是法医物证检案中常用的方法[1],是在模板DNA、引物和四种脱氧核糖核苷酸存在的条件下依赖DNA聚合酶的酶促反应[2];自1985年问世以来,因其特异性、有效性和忠实性在许多方面得到了广泛的应用 ......