二十碳五烯酸抑制肺腺癌A-549细胞株增殖(1)
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[摘要] 目的 在体外细胞实验中，观察二十碳五烯酸(EPA)联合顺铂(DDP)对肺腺癌A-549细胞株的抗增殖和诱导凋亡作用。方法 选用A-549细胞株进行体外培养，用MTT比色法测定EPA对A-549细胞株的抗增殖作用。应用倒置显微镜、光学显微镜观察细胞凋亡形态学变化。应用TUNEL染色法检测细胞凋亡指数(AI)。结果 实验结果表明，EPA在(60～120)μg/mL浓度范围内，于24、48、72、96h四个时间段对肺腺癌A-549细胞株均有抑制生长作用，且EPA与顺铂有协同抗癌作用，并表现出浓度、时间依赖性关系。当EPA浓度为60、80、100、120μg/mL时，细胞凋亡指数(AI)依次升高，分别为(81.52±1.96)%、(83.74±2.21)%、(85.30±2.38)%、(86.26±2.44)%，不同浓度组相比差异有统计学意义(P<0.05)。结论 体外实验中，EPA对A-549细胞生长有抑制作用，呈明显的量效和时效关系，且与顺铂有协同作用。
[关键词] 二十碳五烯酸；增殖；肺腺癌；A-549细胞株
[中图分类号] R734.2 [文献标识码] A [文章编号] 1673-9701(2011)23-07-03
The Effect of Inhibiting Hyperplasia of Eicosapentaenoic Acid on A-549 Human Lung Cancer Cell Line
MA Liujiang AN Jinghua LI Xing SHI Jun
The Affiliated Hospital of Yanbian University, Yanji 133000, China
[Abstract] Objective To observe the effects of anti-proliferation and inducing apoptosis on A-549 cell line induced by eicosapentaenoic acid (EPA) and its synergistic effect with cisplatin (DDP) in vitro. Methods A-549 cell line was cultured in vitro. MTT assay was used to determine the growth inhibitory effect of EPA on A-549 cells. Morphological changes of the apoptosis of A-549 cells were observed by using inverted microscope. TUNEL was used to detect cell cycle distribution and apoptotic rate of A-549 cells. Results The results of experiments showed that when A-549 cells were exposed to EPA for 24h, 48h, 72h and 96h respectively at final concentration of (60-120) μg/mL, the growth of cells was inhibited and showed in dose and time dependent manner. EPA combined with DDP showed significant synergistic anti-tumor effect on A-549 cells. When the concentration of EPA altered from 60μg/mL to 120μg/mL, the apoptotic index (AI) of A-549 cells elevated accordingly by the method of terminal-deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL), the apoptotic indexes were (81.52±1.96)%, (83 ......
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