香烟烟雾对COPD患者支气管肺泡灌洗液SP—D的影响及激素的干预作用研究(1)
[摘要] 目的 研究COPD患者以及吸烟者BALF中SP-D的表达。研究香烟烟雾对Ⅱ型肺泡上皮细胞SP-D表达的影响以及激素对SP-D表达的调节。 方法 (1)实验分为COPD组和对照组，根据是否有嗜烟史又将两组各分为嗜烟组和不嗜烟组。各组患者行支气管镜检查并行支气管肺泡灌洗，检测各组BALF中SP-D的表达。(2)培养人类Ⅱ型肺泡上皮细胞系，分为对照组和香烟烟雾提取物组，对照组：细胞在无血清的培养液中培养。香烟烟雾提取物组：在培养液中加入香烟烟雾提取物培养。12 h 后检测SP-D的表达，然后予地塞米松加入培养液中干预，72 h 后检测培养液中SP-D的水平。 结果 COPD组患者BALF中SP-D较对照组表达明显降低。COPD组中嗜烟组BALF中SP-D明显低于不嗜烟组。对照组中嗜烟组BALF中SP-D明显低于不嗜烟组。体外实验表明，香烟烟雾提取物干预组细胞培养液中SP-D的表达较对照组明显降低，而经地塞米松处理后香烟烟雾提取物干预组细胞培养液中SP-D表达明显增加，对照组经地塞米松处理前后SP-D表达没有明显变化。 结论 COPD患者BALF中SP-D的表达明显降低。嗜烟可明显下调BALF中SP-D的表达。香烟烟雾提取物干预的Ⅱ型肺泡上皮细胞培养液中SP-D的表达明显降低，激素对Ⅱ型肺泡上皮细胞SP-D的表达具有上调作用。
[關键词] 肺表面活性物质D；慢性阻塞性肺疾病；香烟烟雾；糖皮质激素；支气管肺泡灌洗液
[中图分类号] R562.1+2 [文献标识码] A [文章编号] 1673-9701(2017)08-0001-04
[Abstract] Objective To investigate the expression of SP-D in BALF in COPD patients and smokers， and to study the effects of cigarette smoke on the expression of SP-D in type II alveolar epithelial cells and the regulation of SP-D by hormone. Methods (1)The experiment was divided into COPD group and control group， and according to whether there was a history of smoking addiction， they were further divided into smoking addiction group and non-smoking addiction group. Bronchoscopy and bronchoalveolar lavage were performed in all groups. The expression of SP-D in BALF was detected.(2)Human type II alveolar epithelial cell line was cultured， and they were divided into control group and cigarette smoke extract group. Control group： Cells were cultured in serum-free medium. Cigarette smoke extract group： Cigarette smoke extract was added to the culture medium. After 12 hours， the expression of SP-D was detected， and then dexamethasone was added into the culture medium. After 72 hours， the SP-D level in the culture medium was detected. Results The expression of SP-D in BALF in COPD patients was significantly lower than that in the control group. In the COPD group， SP-D in BALF in the smoking addiction group was significantly lower than that in the non-smoking addiction group. In the control group， SP-D in BALF was significantly lower than that in non-smoking addiction group. In vitro experiments showed that the expression of SP-D in the cell culture medium in the cigarette smoke extract intervention group was significantly lower than that in the control group. The expression of SP-D treated with dexamethasone in the cell culture medium in the cigarette smoke extract intervention group was significantly increased. There was no significant change of SP-D expression in the control group before and after dexamethasone treatment. Conclusion The expression of SP-D in BALF was significantly lower in COPD patients. Smoking addiction could down-regulate the expression of SP-D in BALF. The expression of SP-D in type Ⅱ alveolar epithelial cell culture medium treated with cigarette smoke extract is significantly decreased， and hormone up-regulates SP-D expression in type Ⅱ alveolar epithelial cells., 百拇医药(李小波 周杰森 黄华琼 毛立群)
[關键词] 肺表面活性物质D；慢性阻塞性肺疾病；香烟烟雾；糖皮质激素；支气管肺泡灌洗液
[中图分类号] R562.1+2 [文献标识码] A [文章编号] 1673-9701(2017)08-0001-04
[Abstract] Objective To investigate the expression of SP-D in BALF in COPD patients and smokers， and to study the effects of cigarette smoke on the expression of SP-D in type II alveolar epithelial cells and the regulation of SP-D by hormone. Methods (1)The experiment was divided into COPD group and control group， and according to whether there was a history of smoking addiction， they were further divided into smoking addiction group and non-smoking addiction group. Bronchoscopy and bronchoalveolar lavage were performed in all groups. The expression of SP-D in BALF was detected.(2)Human type II alveolar epithelial cell line was cultured， and they were divided into control group and cigarette smoke extract group. Control group： Cells were cultured in serum-free medium. Cigarette smoke extract group： Cigarette smoke extract was added to the culture medium. After 12 hours， the expression of SP-D was detected， and then dexamethasone was added into the culture medium. After 72 hours， the SP-D level in the culture medium was detected. Results The expression of SP-D in BALF in COPD patients was significantly lower than that in the control group. In the COPD group， SP-D in BALF in the smoking addiction group was significantly lower than that in the non-smoking addiction group. In the control group， SP-D in BALF was significantly lower than that in non-smoking addiction group. In vitro experiments showed that the expression of SP-D in the cell culture medium in the cigarette smoke extract intervention group was significantly lower than that in the control group. The expression of SP-D treated with dexamethasone in the cell culture medium in the cigarette smoke extract intervention group was significantly increased. There was no significant change of SP-D expression in the control group before and after dexamethasone treatment. Conclusion The expression of SP-D in BALF was significantly lower in COPD patients. Smoking addiction could down-regulate the expression of SP-D in BALF. The expression of SP-D in type Ⅱ alveolar epithelial cell culture medium treated with cigarette smoke extract is significantly decreased， and hormone up-regulates SP-D expression in type Ⅱ alveolar epithelial cells., 百拇医药(李小波 周杰森 黄华琼 毛立群)