[摘要] 目的 研究活化的蛋白激酶C受体(Receptor for activated protein kinase C，RACK1)与蛋白激酶WEE1互作调控胃癌细胞株HGC27生长增殖及其作用机制。 方法 采用LipofectAMINE 2000在HGC27细胞中过表达RACK1，Western blotting检测HGC27细胞中WEE1蛋白表达；免疫共沉淀验证RACK1和WEE1在HGC27细胞内存在相互作用；细胞内免疫荧光观测RACK1与WEE1在HGC27细胞中的表达定位情况。 结果 过表达RACK1后WEE1在胃癌细胞HGC27中的表达降低，RACK1与WEE1在HGC27细胞内存在相互作用且共定位在细胞质内。结论 RACK1与WEE1共同作用调控胃癌的发生发展过程，为RACK1成为临床上胃癌治疗的潜在靶点提供理论基础和实验依据。

    [关键词] 活化的蛋白激酶C受体；WEE1；胃癌；HGC27细胞

    [中图分类号] R735.2 [文献标识码] A [文章编号] 1673-9701(2017)23-0029-04

    [Abstract] Objective To study the regulatory role of the interaction of scaffolding protein RACK1 with protein kinase WEE1 in the proliferation of gastric cancer cell line HGC27 and the mechanism. Methods RACK1 was overexpressed in HGC27 cells by LipofectAMINE 2000. Western blotting was used to detect the expression of WEE1 protein in HGC27 cells. Immunoprecipitation showed that RACK1 interacted with WEE1 in HGC27 cells. Immunofluorescence was used to observe the expression and positioning of RACK1 and WEE1 in HGC27 cells. Results After expression of RACK1 ......