Lgr5、TFF2在萎缩性胃炎及低级别上皮内瘤变中的表达及临床意义(1)
[摘要] 目的 探讨Lgr5、TFF2在萎缩性胃炎、低级别上皮内瘤变中的表达及临床意义。 方法 随机选取2018年1～6月在湖北省中医院病理科确诊为萎缩性胃炎的胃镜活检蜡块30例、低级别上皮内瘤变的20例，采用免疫组织化学实验检测Lgr5、TFF2蛋白表达情况。 结果 Lgr5、TFF2蛋白表达均为阳性，Lgr5在低级别上皮内瘤变中的表达水平高于萎缩性胃炎，但两组比较，差异无统计学意义(P>0.05);TFF2在低级别上皮内瘤变中的表达水平低于萎缩性胃炎，两组比较，差异有统计学意义(P<0.05)。 结论 Lgr5、TFF2的表达与胃癌前病变的病理严重程度相关，可作为潜在预测胃癌发生、发展、预后的重要生物学标记。
[关键词] Lgr5;TFF2;萎缩性胃炎;低级别上皮内瘤变;胃癌前病变
[中图分类号] R573.3 [文献标识码] A [文章编号] 1673-9701(2019)12-0020-04
Expression and clinical significance of Lgr5 and TFF2 in atrophic gastritis and low-grade intraepithelial neoplasia
WEN Na1 HU Yunlian2
1.Hubei University of Traditional Chinese Medicine， Wuhan 430061， China; 2.Hubei Provincial Hospital of Traditional Chinese Medicine， Wuhan 430061， China
[Abstract] Objective To explore the expression and clinical significance of Lgr5 and TFF2 in atrophic gastritis and low-grade intraepithelial neoplasia. Methods Thirty gastroscopic biopsy waxes diagnosed as atrophic gastritis and 20 waxes of low-grade intraepithelial neoplasia treated in our hospital from January to June 2018 were randomly selected. Immunohistochemistry was used to detect the expression of Lgr5 and TFF2 proteins. Results The expression of Lgr5 and TFF2 protein were positive. The expression level of Lgr5 in low-grade intraepithelial neoplasia was higher than that in atrophic gastritis， but there was no significant difference between the two groups(P>0.05). The expression level of TFF2 in low-grade intraepithelial neoplasia was lower than that in atrophic gastritis， and the difference was significant (P<0.05). Conclusion The expression of Lgr5 and TFF2 is related to the pathological severity of gastric precancerous lesions， and can be used as an important biomarker for predicting the occurrence， development and prognosis of gastric cancer.
[Key words] Lgr5; TFF2; Atrophic gastritis; Low-grade intraepithelial neoplasia; Gastric precancerous lesions
胃癌是世界范圍内高发病率、高死亡率的癌症之一，尽管有先进的诊断工具和治疗方法，其5 年生存率仍低于30%[1]。肠型胃癌的发病是多因素、多步骤的病理过程，目前认可的病理变化是：慢性浅表性胃炎→慢性萎缩性胃炎→肠上皮化生→上皮内瘤变→肠型胃癌，其中萎缩性胃炎是癌前疾病，而肠化、上皮内瘤变是癌前病变。近年来研究发现肿瘤可能起源于干细胞，胃肠道干细胞是存在于胃肠道组织中的成体干细胞，能分化形成各种胃肠道上皮细胞，目前可通过识别细胞表面标志物如CD44、CD133、TFF2、Lgr5等方法研究胃癌及癌前状态的相关分子表达水平。本次实验拟通过检测Lgr5、TFF2在萎缩性胃炎、低级别上皮内瘤变中的表达情况，来推测Lgr5、TFF2蛋白表达与胃癌发生的关系，现报道如下。
1 材料与方法
1.1 标本来源
标本来自病理科2018年1～6月湖北省中医院病理科归档的蜡块，随机法从中选出病理诊断为萎缩性胃炎的病例30例，其中男16例，女14例，年龄25～68岁，平均(51.5±8.13)岁;低级别上皮内瘤变病例20例，其中男10例，女10例，年龄24～73岁，平均(51.7±11.3)岁;每个指标每例1张，共计50*2张，每例标本均经本院资深的病理医师行病理学诊断，病理资料齐全。, 百拇医药(文娜 胡运莲)
[关键词] Lgr5;TFF2;萎缩性胃炎;低级别上皮内瘤变;胃癌前病变
[中图分类号] R573.3 [文献标识码] A [文章编号] 1673-9701(2019)12-0020-04
Expression and clinical significance of Lgr5 and TFF2 in atrophic gastritis and low-grade intraepithelial neoplasia
WEN Na1 HU Yunlian2
1.Hubei University of Traditional Chinese Medicine， Wuhan 430061， China; 2.Hubei Provincial Hospital of Traditional Chinese Medicine， Wuhan 430061， China
[Abstract] Objective To explore the expression and clinical significance of Lgr5 and TFF2 in atrophic gastritis and low-grade intraepithelial neoplasia. Methods Thirty gastroscopic biopsy waxes diagnosed as atrophic gastritis and 20 waxes of low-grade intraepithelial neoplasia treated in our hospital from January to June 2018 were randomly selected. Immunohistochemistry was used to detect the expression of Lgr5 and TFF2 proteins. Results The expression of Lgr5 and TFF2 protein were positive. The expression level of Lgr5 in low-grade intraepithelial neoplasia was higher than that in atrophic gastritis， but there was no significant difference between the two groups(P>0.05). The expression level of TFF2 in low-grade intraepithelial neoplasia was lower than that in atrophic gastritis， and the difference was significant (P<0.05). Conclusion The expression of Lgr5 and TFF2 is related to the pathological severity of gastric precancerous lesions， and can be used as an important biomarker for predicting the occurrence， development and prognosis of gastric cancer.
[Key words] Lgr5; TFF2; Atrophic gastritis; Low-grade intraepithelial neoplasia; Gastric precancerous lesions
胃癌是世界范圍内高发病率、高死亡率的癌症之一，尽管有先进的诊断工具和治疗方法，其5 年生存率仍低于30%[1]。肠型胃癌的发病是多因素、多步骤的病理过程，目前认可的病理变化是：慢性浅表性胃炎→慢性萎缩性胃炎→肠上皮化生→上皮内瘤变→肠型胃癌，其中萎缩性胃炎是癌前疾病，而肠化、上皮内瘤变是癌前病变。近年来研究发现肿瘤可能起源于干细胞，胃肠道干细胞是存在于胃肠道组织中的成体干细胞，能分化形成各种胃肠道上皮细胞，目前可通过识别细胞表面标志物如CD44、CD133、TFF2、Lgr5等方法研究胃癌及癌前状态的相关分子表达水平。本次实验拟通过检测Lgr5、TFF2在萎缩性胃炎、低级别上皮内瘤变中的表达情况，来推测Lgr5、TFF2蛋白表达与胃癌发生的关系，现报道如下。
1 材料与方法
1.1 标本来源
标本来自病理科2018年1～6月湖北省中医院病理科归档的蜡块，随机法从中选出病理诊断为萎缩性胃炎的病例30例，其中男16例，女14例，年龄25～68岁，平均(51.5±8.13)岁;低级别上皮内瘤变病例20例，其中男10例，女10例，年龄24～73岁，平均(51.7±11.3)岁;每个指标每例1张，共计50*2张，每例标本均经本院资深的病理医师行病理学诊断，病理资料齐全。, 百拇医药(文娜 胡运莲)