霍鑫 曹治婷 蔡许超

    [摘要] 目的 分析下肢动脉硬化闭塞症(LEASO)患者不同阶段热休克蛋白70(HSP70)及其磷酸化水平的差异及其临床意义。 方法 收集2017年9月至2019年12月在柳州市人民医院收治的80例下肢动脉硬化闭塞症(LEASO)患者的血样，按踝肱指数(ABI)分为A组(ABI≤0.430)和B组(ABI>0.430)。采用酶联免疫吸附法(ELISA)测定本院患者各阶段HSP70的表达，分析两组HSP70浓度及ABI的差异。同时采集4例正常股动脉标本(正常组)和4例动脉粥样硬化性股动脉标本(病变组)，用免疫印迹法检测HSP70及其磷酸化HSP70(Ser15)、磷酸化HSP70(Ser78)和磷酸化HSP70(Ser82)。对正常组与病变组的蛋白表达水平进行统计学分析。 结果 A组HSP70浓度为(46.731±12.986)ng/mL，ABI为(0.231±0.056)。B组HSP70浓度为(67.104±18.262)ng/mL，ABI为(0.622±0.065)。正常组HSP70蛋白表达及磷酸化水平分别为(0.812±0.131) ng/mL、(0.643±0.107) ng/mL、(0.918±0.206) ng/mL和(0.891±0.168) ng/mL;病變组蛋白表达分别为(0.463±0.072) ng/mL、(0.424±0.081) ng/mL、(0.393±0.065) ng/mL和(0.572±0.101) ng/mL，组间比较差异有统计学意义(P0.430) according to the ankle-brachial index (ABI). Enzyme-linked immunosorbent assay (ELISA) was used to determine the expression of HSP70 in patients in our hospital at various stages. The differences in HSP70 concentration and ABI between the two groups were analyzed. At the same time， four normal femoral artery specimens(normal group) and four atherosclerotic femoral artery specimens(pathological group) were collected. The HSP70 and its phosphorylated HSP70(Ser15) ......