张伟锋 舒艳 万择秋

    [摘要]?目的?探讨血清微RNA(microRNA，miRNA)液体活检在早期卵巢癌诊断中的临床价值。方法?选取2021—2023年湖州市妇幼保健院的22个新鲜冷冻Ⅰ期卵巢癌组织和9个相邻正常卵巢组织用于本研究。使用R软件中的limma软件包分析肿瘤和邻近正常组织之间miRNA的差异表达。利用公开的回顾性血清miRNA测序数据集GSE106817使用Ⅰ期卵巢癌中高表达的miRNA训练Logistic回归模型，使用GSE113486和GSE31568验证模型的准确性。结果?Ⅰ期卵巢癌组织中共筛选出8种高表达miRNA，即miR-182、miR-183、miR-202、miR-205、miR-508、miR-509-3、miR-513B和miR-513C。在训练队列GSE106817中用于诊断卵巢癌的曲线下面积(area?under?the?curve，AUC)为0.89。在验证队列GSE31568和GSE113486中诊断卵巢癌的AUC分别为0.85和0.86。结论?血清miRNA液体活检对早期卵巢癌的诊断有较高的临床价值。

    [关键词]?微RNA；液体活检；早期卵巢癌

    [中图分类号]?R711.75；R446.11?[文献标识码]?A?[DOI]?10.3969/j.issn.1673-9701.2024.16.011

    Clinical?value?of?serum?miRNA?liquid?biopsy?in?the?diagnosis?of?early?ovarian?cancer

    ZHANG?Weifeng，?SHU?Yan，?WAN?Zeqiu

    Department?of?Gynecology?and?Obstetrics，?Huzhou?Maternal?and?Child?Health?Care?Hospital，?Huzhou?313000，?Zhejiang，?China

    [Abstract]?Objective?To?investigate?the?clinical?value?of?serum?microRNA?(miRNA)?liquid?biopsy?in?the?diagnosis?of?early?ovarian?cancer.?Methods?Twenty-two?fresh?and?frozen?stage?I?ovarian?cancer?tissues?and?nine?adjacent?normal?ovarian?tissues?from?Huzhou?Maternal?and?Child?Health?Care?Hospital?from?2021?to?2023?were?selected?for?this?study.?Differential?expression?of?miRNA?between?tumors?and?adjacent?normal?tissues?was?analyzed?using?limma?software?package?in?R?software.?The?published?retrospective?serum?miRNA?sequencing?dataset?GSE106817?was?used?to?train?the?Logistic?regression?model?with?highly?expressed?miRNA?in?stage?Ⅰ?ovarian?cancer ......