李兴周 刘建华 李远珍 黄佳玲

    摘 要：建立了一种分光光度法测定酱油种曲的孢子数的方法，以酱油种曲的湿基孢子数与对应吸光度绘制标准曲线，与传统的血球计数法进行了对比分析。结果表明，湿基孢子数在14～72亿个/g线性关系良好，标准曲线回归方程为y=93.70x-1.53，相关系数R2=0.998 8，精密度实验RSD为1.62%，重复性实验RSD为1.92%，与血球计数法相比，方法间平均相对偏差为6.32%，方法间相对偏差合格率为100%，该方法简便快捷、稳定可靠、重复性好。分光光度法能够替代血球计数法用于测定酱油种曲的孢子数。

    关键词：孢子数测定；种曲孢子数；方法对比；分光光度法

    Abstract： An method to determinate the spore number of soy sauce koji by spectrophotometry was established， and the standard curve was drawn based on the spore number of wet-based of soy sauce koji and the corresponding absorbance， and compared with the traditional blood cell counting method. The standard curve had an excellent linearity in the spore number of wet-based of 1.4～7.2 billion spores/g， the regression equation of the standard curve is y＝93.70x-1.53 ......