李莲 林洁娜 罗雪婷

    摘 要：建立高效液相色谱法检测婴幼儿配方奶粉与保健食品中维生素B12的分析方法。选择SB-AQ柱(4.6 mm×250 mm，5.0 μm)作为色谱柱，流动相为0.025%三氟乙酸溶液+乙腈，流速1.0 mL·min-1，梯度洗脱，进样量100 μL，检测波长361 nm。保健品：B族维生素片等样品采用水提取的方式；基质复杂的样品用固相萃取的方法。婴幼儿配方奶粉：含量<4 μg/100 g的样品用固相萃取法，含量≥4 μg/100 g的样品用直接提取方式，经高效液相色谱仪分析。结果表明，维生素B12标准溶液线性良好，r2>0.999，加标回收率在86.2%～96.0%，RSD为1.3%～1.8%，检出限为0.4 μg/100 g。该方法简便快捷，所用试剂耗材少，灵敏度高，结果准确。

    关键词：高效液相色谱法；维生素B12；婴幼儿配方奶粉；保健食品

    Abstract： To establish a low-cost and efficient HPLC method for the determination of vitamin B12 in infant formula milk powder and health food.SB-AQ column (4.6 mm×250 mm， 5.0 μm) was selected as the chromatographic column with mobile phase consisting of 0.025% trifluoroacetic acid solution + acetonitrile at the flow rate of 1.0 mL·min-1. The sample size was 100 μL and the detection wavelength was 361 nm. Health care products： samples such as B vitamin tablets are extracted by water; samples with complex matrix are extraction method. Infant formula milk powder： sample content<4 μg/100 g with solid phase extraction method ......