Determination of Diflorasone Propionate in Health Products byUltra PerformanceLiquid Chromatography Tandem Mass Spectrometry

    MA Xin， WU Junfa， PAN Zhiqi， QIN Yuxin (Guangzhou Inspectionand Testing Certification Group Co.，Ltd.，National Quality Inspection and Testing Center for Processed Food (Guangdong)， Guangzhou 511447， China)

    Abstract： Objective： To establish an ultra-performance liquid chromatography-tandem mass spectrometry method for the determination of diflurazone-17-propionateand diflulazone-2l-propionate in health products. Method： The samples were extracted by ultrasonication or vortexing of acetonitrile and 70% acetonitrile， eluted and separated by Waters BEH- ?C₁₈ column (100mm×2.1mm，3.0μm) ， and then determined by electrospray ionization source.Result： The linear relationship between the two diflularsone propionate esters was good in the range of 2.0～ 100.0μg?L^-1 ，and the correlation coefficient R²?0.9987. ，and the average recoveries were 72.3%～92.3% and the relative standard deviations were 4.43%～8.09% under the three spike levels.The limit of detection of solid tablets was 15μg?kg^-1 and the limit of quantification was 40μg?kg^-1 . The limit of detection and quantification of oil-based capsules was 30μg?kg^-1 and 100μg?kg^-1 .Conclusion： This method has short time ......