响应面优化蜂花粉可溶性膳食纤维提取工艺
液料,1材料与仪器,2方法,3结果与分析,4讨论
郑慧,陈希平*,常新利,孔德凤,陈珍珍,陈娟(湖南中医药大学药学院,湖南长沙410208)·中药制剂与分析·
响应面优化蜂花粉可溶性膳食纤维提取工艺
郑慧,陈希平*,常新利,孔德凤,陈珍珍,陈娟
(湖南中医药大学药学院,湖南长沙410208)
为充分利用蜂花粉资源,采用酸提工艺提取蜂花粉可溶性膳食纤维,利用单因素试验和响应面优化法对液料比、浸提pH值、浸提温度、浸提时间工艺条件进行分析与优化,并测定蜂花粉可溶性膳食纤维的理化指标。结果表明,蜂花粉可溶性膳食纤维酸提最优工艺为:液料比10.8∶1(mL/g)、浸提pH值4.3、浸提温度50℃、浸提时间1.9 h,在此工艺条件下蜂花粉可溶性膳食纤维得率为7.31%,膨胀力0.87 mL/g、持水力1.33 g/g、水溶性84.78%。
蜂花粉;可溶性膳食纤维;响应面优化;提取工艺
〔Abstract〕In order to fully utilize bee pollen resource,the soluble dietary fiber from bee pollen was extracted using acid extraction process.The process conditions of liquid-to-solid,extraction pH,extraction temperature,extraction time were analyzed and optimized by single-factor experiment and response surface methodology.The physicochemical properties of bee pollen soluble dietary fiber were measured.The optimal extraction conditions were determined as follows 10.8:1(mL/g)of liquid-to-solid,pH4.3,extraction temperature 50℃,extraction time 1.9 h.Under the conditions,the yield of bee pollen soluble dietary fiber was 7.31%,swelling capacity was 0.87 mL/g,water holding capacity was 1.33 g/g ......
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