张 驰，夏新华*，周莉莉，李 朝，周 宇

    (湖南中医药大学药学院，湖南 长沙 410208)

    ·文献综述·

    两种纯化工艺对大黄水提液中蒽醌类成分的影响研究

    张驰，夏新华*，周莉莉，李朝，周宇

    (湖南中医药大学药学院，湖南 长沙 410208)

    目的 探索壳聚糖絮凝澄清工艺与醇沉工艺对大黄水提液中蒽醌类成分的影响规律。方法 以大黄中5种蒽醌类成分的保留率为评价指标，考察两种纯化工艺在不同条件下对大黄水提液中蒽醌类成分的影响。结果 两种纯化工艺在不同的条件下，大黄水提液中5种蒽醌类成分的保留率大小几乎具有一致的规律，即大黄酸>芦荟大黄素>大黄素>大黄酚>大黄素甲醚，醇沉工艺对于大黄蒽醌类成分的保留率优于壳聚糖絮凝澄清工艺。结论 醇沉工艺对极性小的成分的保留优于壳聚糖絮凝澄清工艺，而对极性高的成分的保留则相反。

    大黄；蒽醌类；壳聚糖；絮凝澄清；醇沉

    〔Abstract〕Objective To explore the effect laws of chitosan flocculation clarification and alcohol precipitation process on the anthraquinones in aqueous extract solution of Rheum palmatum.Methods The retention rates of five anthraquinones in Rheum palmatum are as evalution indexes，the influences of two purification processes under the different conditions on the anthraquinones were investigated.Results Under the different conditions of two purification processes，the retention rates of five anthraquinones in aqueous extract solution of Rheum palmatum almost had the same laws：rhein>aloe emodin>emodin>chrysophanol>emodin methyl ether.Comparing two purification processes，the retention rates of alcohol precipitation process on anthraquinones were superior to ones of chitosan flocculation clarification process.Conclution The retention rates of low polarity constituents by alcohol precipitation were better than the chitosan flocculation clarification process ......