[关键词]谷胱甘肽；荧光探针；姜黄素；线粒体；活性氧；细胞成像 [中图分类号]R284;R285.5 [文献标志码]A [文章编号]doi：10.3969/j.issn.1674-070X.2025.06.006

    Construction and cell imaging of GSH-responsive curcumin probe

    YANG Haoyu， ZHOU Xinyi， CHEN Xin， DAI Hongfang， QIN Yan* School ofPharmacy，Hunan University of Chinese Medicine， Changsha，Hunan 4lO2O8，China

    [Abstract]ObjectiveTosynthesize a glutathione (GSH)-responsivecurcumin-based fluorescent probe (CPB)andapply it forhepatocellarcarcinomacellimaging，investigatingitscytotoxcitymitochondria-argetingcapability，andabilitytoinduce reactiveoxygen species (ROS)generation Methods A GSH-responsive fluorescent probe (CPB)was synthesized using curcuminasa fluorescentmoietyanditsstructurewascharacterizedbyliquidchromatographyassspectrometry(C-MS)anduclearmagnetic resonance (NMR).Ultraviolet (UV)spectroscopywasused toanalyzeitsresponsetoGSHandtimedependentchanges.Thecytotoxicity ofCPBwasaessdbsaynditsgetielodioiaonicellagingdhodalcalatio experiment.Results Theresultsof NMRcarbon spectraandLC-MSconfirmed thesuccesful synthesisof CPB.UVspectra showedthattheresponsesignalofCPBitself wasconcentration-dependent ......