[关键词]肺腺癌；小泻肺汤；网络药理学;肿瘤蛋白53;蛋白激酶B;原癌基因;肿瘤坏死因子；热休克蛋白 90α [中图分类号]R285.5 [文献标志码]A [文章编号]doi：10.3969/j.issn.1674-070X.2025.06.026

    Mechanism of Xiao Xiefei Decoction on lung adenocarcinoma based on network pharmacology and in vitro experimental validation

    LIU Yanxia'， GU Zhancheng'，YANG Hua'， LU Qiong'， QIAN Lijun'，YANG Wenjuan ^I* ， CAO Hong'* Kunshan Hospital of Chinese Medicine， Suzhou， Jiangsu 2153o0， China

    [Abstract]ObjectiveTo explore thepotential targetsand mechanismsof Xiao Xiefei Decoction(XXFD)inthetreatmentof lungadenocarcinomabasedonnetworkpharmacologyfollowedbyinvivoexperimentalvalidationMethodsEfectivechemical componentsofXXFDandtheircorrespondingtargetswereobtainedfrom TCMSPandUniProtdatabases，aswellasliterature sources.Lungadenocarcinoma-related targets wereidentifiedusingthe GeneCardsandOMIM databases.Venny2.1.0wasusedto determinetheoverlapping targetsbetween thedrugandthedisease，representingthepotentialtargetsofXXFDintreatinglung adenocarcinomaAprotein-proteininteraction(PPI networkwasconstructedusingSTRINGdatabaseandCytoscape3.9.1softwareto identifykeytargets.GOfunctionalandKEGGenrichmentanalysesoftheintersecting targetswereperformedusingDAVID database.Acomponent-target-pathwaynetworkof XXFDinthetreatment oflungadenocarcinoma wasconstructedusing Cytoscape 3.9.lsoftware.Aninvivosubcutaneous xenograft tumor model wasestablishedinnudemice ......