慢性应激对大鼠胸腺指数和血清CORT、胸腺肽浓度的影响(1)
[摘要] 目的 研究慢性应激对大鼠胸腺指数和血清糖皮质激素(CORT)和胸腺肽浓度的影响。方法 大鼠随机分为对照组和应激组。应激组大鼠给予复合应激，即禁水12 h、禁食12 h、束缚6 h、昼夜颠倒、强迫游泳15 min、潮湿垫料24 h和45°浸水5 min7种应激刺激，每天随机一次，7 d为一个周期，每种刺激应用一次，共14 d。应激结束后取材，计算胸腺指数，酶联免疫吸附测定法检测血清CORT和胸腺肽的含量。 结果 应激组大鼠胸腺指数为(1.08±0.13)mg/g，与对照组[(1.45±0.11)mg/g]比较显著性减低，有统计学意义(P<0.05)；应激组大鼠血清CORT浓度为(87.91±2.45) pg/mL，与对照组[(77.28±1.56)pg/mL]比较明显增高，有统计学意义；应激组大鼠血清胸腺肽的含量为(0.93±0.03)×103 pg/mL，与对照组[(1.40±0.06)×103 pg/mL]比较显著性降低，有统计学意义(P<0.05)。结论 慢性应激会降低大鼠胸腺指数，抑制胸腺肽的分泌功能。
[关键词] 慢性应激；胸腺；ACTH；胸腺肽
[中图分类号] R4 [文献标识码] A [文章编号] 1674-0742(2015)09(a)-0096-02
Effects of Chronic Stress on Thymus Index and Concentration of Serum CORT and Thymosin in Rats
CUIChun-hong1， SUN Zhao-xia2， LI Wei1， JIANG He1
1. Department of Histology and Embryology， Shandong Wanjie Medical School， Zibo， Shandong Province， 255213 China； 2. Department of Anatomy， Shandong Wanjie Medical School， Zibo， Shandong Province， 255213 China
[Abstract] Objective To To study the influence of acute stress on thymus index and concentration of serum CORT and thymosin in rats. Methods Rats were randomly divided into control group and stress group. Stress group rats were given compound stress， including absolute diet 12 h， water deprivation 12 h， being bounded 6 h， day and night inversion， forced swimming 15 min， damp bedding 24 h， 45° water immersion， which were randomly given once a day with 7 days for a cycle， and each stimulus was applied once for a total of 14 days. Then rats were killed and serum CORT and thymosin were extracted. We calculated the thymus index and determined the concentration of serum CORT and thymopeptide using enzyme-linked immunosorbent assay. Results The thymus index was (1.08±0.13)mg/g in the stress group ， lower than (1.45±0.11)mg/g in the control group， and the difference was statistically significant， P<0.05. The CORT levels was (87.91±2.45) pg/ml in the stress group， higher than (77.28±1.56)pg/ml in the control group， and the difference was statistically significant， P<0.05； the concentration of serum thymosin was (0.93±0.03)×103 pg/ml in the stress group， lower than(1.40±0.06)×103pg/ml in the control group， and the difference was statistically significant， P<0.05. Conclusion Chronic stress can lower the thymus index and inhibit the secretion of the thymosin in rats.
[Key words] Chronic Stress； Thymus； ACTH； Thymosin
应激是个体面临或察觉到环境变化对机体有威胁或挑战时所做出的适应和应对过程。长时间或过强的应激可引起神经、内分泌失调，免疫功能障碍[1]，而机体对应激的反应主要表现在交感-肾上腺髓质轴(sympathetic-adrenal medullary，SAM)高度兴奋和下丘脑-垂体-肾上腺皮质轴(hypothalamic-pituitary-adrenal axis，HPA)反应，引起神经内分泌紊乱[2]。胸腺是机体重要的中枢免疫器官，是T淋巴细胞发育、成熟的场所，对机体外周淋巴器官的发育和免疫应答的发生起着重要的调节作用[3]。应激反应可导致胸腺的退化萎缩[4]，并伴有免疫功能受损。目前，对应激反应中血清胸腺肽浓度的研究较少。本实验试图通过慢性应激探讨应激对大鼠胸腺指数和血清CORT以及胸腺肽浓度的影响，为进一步探讨应激影响机体免疫功能的机制与途径奠定基础。测定胸腺指数意在了解应激对大鼠胸腺体积、重量的影响；检测应激后血清胸腺肽浓度，旨在了解应激对胸腺肽的影响，为进一步探讨胸腺肽在应激影响胸腺结构过程中的作用及其机制提供理论依据，现报道如下。, http://www.100md.com(崔春红 等)
[关键词] 慢性应激；胸腺；ACTH；胸腺肽
[中图分类号] R4 [文献标识码] A [文章编号] 1674-0742(2015)09(a)-0096-02
Effects of Chronic Stress on Thymus Index and Concentration of Serum CORT and Thymosin in Rats
CUIChun-hong1， SUN Zhao-xia2， LI Wei1， JIANG He1
1. Department of Histology and Embryology， Shandong Wanjie Medical School， Zibo， Shandong Province， 255213 China； 2. Department of Anatomy， Shandong Wanjie Medical School， Zibo， Shandong Province， 255213 China
[Abstract] Objective To To study the influence of acute stress on thymus index and concentration of serum CORT and thymosin in rats. Methods Rats were randomly divided into control group and stress group. Stress group rats were given compound stress， including absolute diet 12 h， water deprivation 12 h， being bounded 6 h， day and night inversion， forced swimming 15 min， damp bedding 24 h， 45° water immersion， which were randomly given once a day with 7 days for a cycle， and each stimulus was applied once for a total of 14 days. Then rats were killed and serum CORT and thymosin were extracted. We calculated the thymus index and determined the concentration of serum CORT and thymopeptide using enzyme-linked immunosorbent assay. Results The thymus index was (1.08±0.13)mg/g in the stress group ， lower than (1.45±0.11)mg/g in the control group， and the difference was statistically significant， P<0.05. The CORT levels was (87.91±2.45) pg/ml in the stress group， higher than (77.28±1.56)pg/ml in the control group， and the difference was statistically significant， P<0.05； the concentration of serum thymosin was (0.93±0.03)×103 pg/ml in the stress group， lower than(1.40±0.06)×103pg/ml in the control group， and the difference was statistically significant， P<0.05. Conclusion Chronic stress can lower the thymus index and inhibit the secretion of the thymosin in rats.
[Key words] Chronic Stress； Thymus； ACTH； Thymosin
应激是个体面临或察觉到环境变化对机体有威胁或挑战时所做出的适应和应对过程。长时间或过强的应激可引起神经、内分泌失调，免疫功能障碍[1]，而机体对应激的反应主要表现在交感-肾上腺髓质轴(sympathetic-adrenal medullary，SAM)高度兴奋和下丘脑-垂体-肾上腺皮质轴(hypothalamic-pituitary-adrenal axis，HPA)反应，引起神经内分泌紊乱[2]。胸腺是机体重要的中枢免疫器官，是T淋巴细胞发育、成熟的场所，对机体外周淋巴器官的发育和免疫应答的发生起着重要的调节作用[3]。应激反应可导致胸腺的退化萎缩[4]，并伴有免疫功能受损。目前，对应激反应中血清胸腺肽浓度的研究较少。本实验试图通过慢性应激探讨应激对大鼠胸腺指数和血清CORT以及胸腺肽浓度的影响，为进一步探讨应激影响机体免疫功能的机制与途径奠定基础。测定胸腺指数意在了解应激对大鼠胸腺体积、重量的影响；检测应激后血清胸腺肽浓度，旨在了解应激对胸腺肽的影响，为进一步探讨胸腺肽在应激影响胸腺结构过程中的作用及其机制提供理论依据，现报道如下。, http://www.100md.com(崔春红 等)