HPLC-ELSD法测定银杏叶茶中总内酯的含量(1)
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2011年2月15日
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[摘要] 目的：采用RP-HPLC法测定低酚酸银杏叶保健茶中总内酯的含量。方法：采用Agilent C18(4.6 mm×250 mm，5 μm)色谱柱，以甲醇-四氢呋喃-水(25∶10∶65)为流动相，柱温为30℃，蒸发光散射检测器检测。结果：银杏叶保健茶中银杏内酯A、银杏内酯B、银叶内酯C和白果内酯的进样量的对数与峰面积的对数线性关系较好，线性范围分别为0.485～9.701 μg、0.460～9.204 μg、0.501～11.899 μg、0.935～18.703 μg；银杏内酯A、银杏内酯B、银叶内酯C和白果内酯加样回收率分别为100.45%(RSD为2.79%)、98.29%(RSD为2.90%)、98.22%(RSD为2.53%)、101.32%(RSD为1.81%)。结论：本方法简便、准确、分离效果好，适合于银杏叶茶总内酯的含量测定。
[关键词] 银杏叶茶；总内酯；含量测定
[中图分类号] R927.2 [文献标识码] C[文章编号] 1674-4721(2011)02(b)-040-03
Determination of Ginkgolides in teas of Ginkgo biloba by HPLC-ELSD
SONG Weifeng1, LI Ruiming2
(1.The Third Affiliated Hospital of Sun Yat-sen University, Guangdong Province, Guangzhou 510630, China; 2.The First Affiliated Hospital of Sun-Sen University, Guangdong Province, Guangzhou 510080, China)
[Abstract] Objective: To establish an RP-HPLC method for quantitative determination of Ginkgolides in teas of Ginkgo biloba. Methods: The evaporation light scattering detector(ELSD) were employed. The analytical column was C18 column (4.6 mm× 250 mm, 5 μm). The mobile phase was composed of methanol-tetrahydrofuran-water(25∶10∶65) at a flow rate of 1.0 ml/min. Results: The logarithm value of sample size had a good linear relationship with the logarithm value of peak area when the sample size of ginkgolide A,B,C and bilobalide was 0.485～9.701 μg, 0.460～9.204 μg, 0.501～11.899 μg, 0.935～18.703 μg respectively. The average recovery was 100.45%(RSD=2.79%), 98.29%(RSD=2.90%), 98.22%(RSD=2.53%), 101.32% (RSD=1.81%) respectively. Conclusion: This method is sensitive, repeatable and suitable to determine the content of total Ginkgolides in teas of Ginkgo biloba.
[Key words] Teas of Ginkgo biloba; Ginkgolides; Determination
银杏叶保健茶是由市售银杏叶经过净选、洗净、烘干、粉碎及超临界二氧化碳萃取、分装等工序制备出的袋泡茶剂[1]。脱酸银杏叶茶中主要含有黄酮类化合物和银杏萜类内酯两类生物活性物[2]。其中萜类内酯成分主要以银杏内酯A(C20H24O9)、银杏内酯B、(C20H24O10)、银杏内酯C(C20H24O11)和白果内酯(C15H18O8)为主。经各国学者对其药理作用进行多年研究发现银杏内酯为特异性血小板活化子(PAF)的强拮抗药。PAF是一种内源性磷脂，由血小板和多种炎症细胞产生、分泌，与许多疾病的发生、发展密切相关。而银杏内酯为目前天然PAF受体拮抗药中最具用前景的药物[3]。另外，已有研究证实，银杏苦内酯还能影响脑的调节功能，可改善与年龄相关的记忆力减退[4]。目前银杏叶每年消耗量已经达10多万吨[5]，具有很高的药用与食用价值[6]。本文用HPLC方法测定总内酯的含量，为建立银杏叶茶质量控制方法提供依据。
1 仪器与试剂
WATERS 2695/2996(ailliance)、色谱柱Agilent C18(250 mm ×4 ......
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